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使用抗氧化剂对厌氧菌进行需氧培养:初步报告。

Aerobic culture of anaerobic bacteria using antioxidants: a preliminary report.

作者信息

La Scola B, Khelaifia S, Lagier J-C, Raoult D

机构信息

Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198, Inserm 1095, Faculté de médecine, Aix Marseille Universite, 27 Boulevard Jean Moulin, 13385, Marseille, Cedex 05, France.

出版信息

Eur J Clin Microbiol Infect Dis. 2014 Oct;33(10):1781-3. doi: 10.1007/s10096-014-2137-4. Epub 2014 May 13.

DOI:10.1007/s10096-014-2137-4
PMID:24820294
Abstract

Antioxidants have been shown to help the growth of anaerobic bacteria. We were able to grow six anaerobe species (including Fusobacterium necrophorum and Ruminococcus gravus) and seven aerobic species all aerobically in Schaedler agar tubes and agar plates with high doses of ascorbic acid and/or glutathione. This may deeply change strategies for culturing bacteria.

摘要

抗氧化剂已被证明有助于厌氧菌的生长。我们能够在含有高剂量抗坏血酸和/或谷胱甘肽的 Schaedler 琼脂试管和琼脂平板中,使六种厌氧菌(包括坏死梭杆菌和沉重瘤胃球菌)和七种需氧菌在有氧条件下生长。这可能会深刻改变细菌培养策略。

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Anaerobe. 2013 Aug;22:123-5. doi: 10.1016/j.anaerobe.2013.04.011. Epub 2013 Apr 29.
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Identification of rare pathogenic bacteria in a clinical microbiology laboratory: impact of matrix-assisted laser desorption ionization-time of flight mass spectrometry.临床微生物学实验室中罕见病原菌的鉴定:基质辅助激光解吸电离飞行时间质谱的影响。
J Clin Microbiol. 2013 Jul;51(7):2182-94. doi: 10.1128/JCM.00492-13. Epub 2013 May 1.
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Axenic culture of fastidious and intracellular bacteria.
Microorganisms. 2023 Apr 20;11(4):1080. doi: 10.3390/microorganisms11041080.
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Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques.培养人类口腔微生物群:更新方法和培养技术
Microorganisms. 2023 Mar 24;11(4):836. doi: 10.3390/microorganisms11040836.
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Using Plate-Wash PCR and High-Throughput Sequencing to Measure Cultivated Diversity for Natural Product Discovery Efforts.使用平板洗涤PCR和高通量测序来测量用于天然产物发现工作的培养多样性。
Front Microbiol. 2021 Jul 20;12:675798. doi: 10.3389/fmicb.2021.675798. eCollection 2021.
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Comprehensive Cultivation of the Swine Gut Microbiome Reveals High Bacterial Diversity and Guides Bacterial Isolation in Pigs.猪肠道微生物群的综合培养揭示了高度的细菌多样性并指导猪的细菌分离
mSystems. 2021 Aug 31;6(4):e0047721. doi: 10.1128/mSystems.00477-21. Epub 2021 Jul 20.
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