Sudan Rasleen, Bhagat Madhulika, Gupta Sahil, Singh Jasvinder, Koul Anupurna
School of Biotechnology, University of Jammu, Jammu, Jammu and Kashmir 180006, India.
Department of Pharmacology, Indian Institute of Integrative Medicine, Jammu, Jammu and Kashmir 180001, India.
Biomed Res Int. 2014;2014:179865. doi: 10.1155/2014/179865. Epub 2014 May 6.
This study explored the antioxidant and immunomodulatory potential of ethnomedicinally valuable species, namely, Arisaema jacquemontii of north-western Himalayan region. The tubers, leaves, and fruits of this plant were subjected to extraction using different solvents. In vitro antioxidant studies were performed in terms of chelation power on ferrous ions and FRAP assay. The crude methanol extract of leaves was found to harbour better chelating capacity (58% at 100 μ g/mL) and reducing power (FRAP value 1085.4 ± 0.11 μ MFe(3+)/g dry wt.) than all the other extracts. The crude methanol extract was thus further partitioned with solvents to yield five fractions. Antioxidant study of fractions suggested that the methanol fraction possessed significant chelation capacity (49.7% at 100 μ g/mL) and reducing power with FRAP value of 1435.4 μ M/g dry wt. The fractions were also studied for immune modulating potential where it was observed that hexane fraction had significant suppressive effect on mitogen induced T-cell and B-cell proliferation and remarkable stimulating effect on humoral response by 141% and on DTH response by 168% in immune suppressed mice as compared to the controls. Therefore, it can be concluded that A. jacquemontii leaves hold considerable antioxidant and immunomodulating potential and they can be explored further for the identification of their chemical composition for a better understanding of their biological activities.
本研究探索了喜马拉雅西北部地区具有民族药用价值的物种——藏南天南星的抗氧化和免疫调节潜力。对该植物的块茎、叶片和果实使用不同溶剂进行提取。体外抗氧化研究通过亚铁离子螯合能力和铁还原抗氧化能力（FRAP）测定来进行。结果发现，叶片的粗甲醇提取物比所有其他提取物具有更好的螯合能力（100μg/mL时为58%）和还原能力（FRAP值为1085.4±0.11μMFe(3+)/g干重）。因此，将粗甲醇提取物进一步用溶剂进行分离，得到五个馏分。馏分的抗氧化研究表明，甲醇馏分具有显著的螯合能力（100μg/mL时为49.7%）和还原能力，FRAP值为1435.4μM/g干重。还对馏分的免疫调节潜力进行了研究，结果观察到，与对照组相比，在免疫抑制小鼠中，己烷馏分对丝裂原诱导的T细胞和B细胞增殖具有显著抑制作用，对体液反应有显著刺激作用，刺激率为141%，对迟发型超敏反应的刺激率为168%。因此，可以得出结论，藏南天南星叶片具有相当大的抗氧化和免疫调节潜力，可以进一步探索其化学成分，以便更好地了解其生物活性。
