Espanha Lívia Greghi, Resende Flávia Aparecida, de Sousa Lima Neto José, Boldrin Paula Karina, Nogueira Catarine Haidê, de Camargo Mariana Santoro, De Grandis Rone Aparecido, dos Santos Lourdes Campaner, Vilegas Wagner, Varanda Eliana Aparecida
Department of Biological Sciences, Faculty of Pharmaceutical Sciences of Araraquara, UNESP- São Paulo State University, Rodovia Araraquara-Jaú, km 1, 14801-902 Araraquara, São Paulo, Brazil.
BMC Complement Altern Med. 2014 Jun 5;14:182. doi: 10.1186/1472-6882-14-182.
In various regions of Brazil, several species of the genus Byrsonima (Malpighiaceae) are widely used to treat gastrointestinal complications. This genus has about 150 species of shrubs and trees distributed over the entire Neotropical region. Various biological activities have been identified in these plants, especially antioxidant, antimicrobial and topical and systemic anti-inflammatory activities. The aim of this study was to investigate the mutagenicity and antimutagenicity of hydroalcoholic leaf extracts of six species of Byrsonima: B. verbascifolia, B. correifolia, B. coccolobifolia, B. ligustrifolia, B. fagifolia and B. intermedia by the Salmonella microsome assay (Ames test).
Mutagenic and antimutagenic activity was assessed by the Ames test, with the Salmonella typhimurium tester strains TA100, TA98, TA97a and TA102, with (+S9) and without (-S9) metabolization, by the preincubation method.
Only B. coccolobifolia and B. ligustrifolia showed mutagenic activity. However, the extracts of B. verbascifolia, B. correifolia, B. fagifolia and B. intermedia were found to be strongly antimutagenic against at least one of the mutagens tested.
These results contribute to valuable data on the safe use of medicinal plants and their potential chemopreventive effects. Considering the excellent antimutagenic activities extracted from B. verbascifolia, B. correifolia, B. fagifolia and B. intermedia, these extracts are good candidate sources of chemopreventive agents. However, B. coccolobifolia and B. ligustrifolia showed mutagenic activity, suggesting caution in their use.
在巴西的不同地区，多种叶下珠属（金虎尾科）植物被广泛用于治疗胃肠道并发症。该属约有150种灌木和乔木，分布于整个新热带地区。这些植物具有多种生物活性，尤其是抗氧化、抗菌以及局部和全身抗炎活性。本研究的目的是通过沙门氏菌微粒体试验（艾姆斯试验），调查六种叶下珠属植物：毛叶叶下珠、心叶叶下珠、海葡萄叶叶下珠、女贞叶叶下珠、锥叶叶下珠和中间叶下珠的水醇叶提取物的致突变性和抗突变性。
采用预孵育法，通过艾姆斯试验，使用鼠伤寒沙门氏菌测试菌株TA100、TA98、TA97a和TA102，在有（+S9）和无（-S9）代谢活化的情况下，评估致突变和抗突变活性。
只有海葡萄叶叶下珠和女贞叶叶下珠表现出致突变活性。然而，发现毛叶叶下珠、心叶叶下珠、锥叶叶下珠和中间叶下珠的提取物对至少一种测试诱变剂具有很强的抗突变性。
这些结果为药用植物的安全使用及其潜在的化学预防作用提供了有价值的数据。鉴于从毛叶叶下珠、心叶叶下珠、锥叶叶下珠和中间叶下珠中提取的优异抗突变活性，这些提取物是化学预防剂的良好候选来源。然而，海葡萄叶叶下珠和女贞叶叶下珠表现出致突变活性，表明在使用时需谨慎。
