Li Yong, Zheng Ming-Hui, Wang Hai-Ming, Lin Cai-Li, Wang Xi-Zhuo
1The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
2Forest Protection Station of Heze, Heze, Shandong province, PR China.
Int J Syst Evol Microbiol. 2019 Jan;69(1):63-67. doi: 10.1099/ijsem.0.003077. Epub 2018 Nov 7.
A Gram-stain-negative, facultatively anaerobic, motile bacterial strain, designated gBX10-1-2, was isolated from symptomatic bark of Populus×euramericana canker in China. Phylogenetic analysis based on its 16S rRNA gene sequence showed that the novel isolate belonged to the genus Brenneria, and shared the highest sequence similarity to Brenneria nigrifluens LMG 2694 (98.3 %). In the phylogenetic trees based on the four housekeeping genes sequences, the novel strain formed a separate branch different from B. nigrifluens LMG 2694, indicating that the novel strain should be classified as a novel species. The genome sequence-derived average nucleotide identity (ANI) values between the novel isolate and B. nigrifluens LMG 2694, Brenneria roseaesubsp. roseae FRB 222 and Brenneria roseaesubsp. americana FRB 223 were less than 85 %, lower than the proposed species boundary ANI cut-off value (95-96 %). The DNA G+C content was 56.2 mol%, and the main fatty acids were C16 : 0, C16 : 1ω7c, C18 : 1ω7c and C17 : 0cyclo. Based on the phenotypic and genotypic characteristics, strain gBX10-1-2 represents a novel species of genus Brenneria, for which the name Brenneria corticis sp. nov. is proposed. The type strain is gBX10-1-2 (=CFCC 11842=KCTC 42840).
从中国欧美杨溃疡病有症状的树皮中分离出一株革兰氏染色阴性、兼性厌氧、具运动性的细菌菌株，命名为gBX10-1-2。基于其16S rRNA基因序列的系统发育分析表明，该新分离菌株属于布伦纳氏菌属，与黑流布伦纳氏菌LMG 2694的序列相似性最高（98.3%）。在基于四个管家基因序列构建的系统发育树中，该新菌株形成了一个与黑流布伦纳氏菌LMG 2694不同的独立分支，表明该新菌株应被归类为一个新物种。该新分离菌株与黑流布伦纳氏菌LMG 2694、玫瑰布伦纳氏菌玫瑰亚种FRB 222和玫瑰布伦纳氏菌美洲亚种FRB 223之间基于基因组序列的平均核苷酸同一性（ANI）值均低于85%，低于提议的物种界限ANI临界值（95-96%）。DNA G+C含量为56.2 mol%，主要脂肪酸为C16:0、C16:1ω7c、C18:1ω7c和C17:0环丙烷。基于表型和基因型特征，菌株gBX10-1-2代表布伦纳氏菌属的一个新物种，为此提议将其命名为树皮布伦纳氏菌（Brenneria corticis sp. nov.）。模式菌株为gBX10-1-2（=CFCC 11842=KCTC 42840）。
