Zhou Yizhuang, Pope Phillip B, Li Shaochun, Wen Bo, Tan Fengji, Cheng Shu, Chen Jing, Yang Jinlong, Liu Feng, Lei Xuejing, Su Qingqing, Zhou Chengran, Zhao Jiao, Dong Xiuzhu, Jin Tao, Zhou Xin, Yang Shuang, Zhang Gengyun, Yang Huangming, Wang Jian, Yang Ruifu, Eijsink Vincent G H, Wang Jun
1] Shenzhen Key laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, CHINA [2] BGI-Shenzhen, Shenzhen 518083, CHINA [3].
1] Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, 1432 NORWAY [2].
Sci Rep. 2014 Jun 13;4:5288. doi: 10.1038/srep05288.
Reaching a comprehensive understanding of how nature solves the problem of degrading recalcitrant biomass may eventually allow development of more efficient biorefining processes. Here we interpret genomic and proteomic information generated from a cellulolytic microbial consortium (termed F1RT) enriched from soil. Analyses of reconstructed bacterial draft genomes from all seven uncultured phylotypes in F1RT indicate that its constituent microbes cooperate in both cellulose-degrading and other important metabolic processes. Support for cellulolytic inter-species cooperation came from the discovery of F1RT microbes that encode and express complimentary enzymatic inventories that include both extracellular cellulosomes and secreted free-enzyme systems. Metabolic reconstruction of the seven F1RT phylotypes predicted a wider genomic rationale as to how this particular community functions as well as possible reasons as to why biomass conversion in nature relies on a structured and cooperative microbial community.
全面了解自然界如何解决顽固性生物质降解问题，最终可能会推动更高效生物精炼工艺的发展。在此，我们解读了从土壤中富集的纤维素分解微生物群落（称为F1RT）产生的基因组和蛋白质组信息。对F1RT中所有七种未培养系统发育型的重建细菌草图基因组分析表明，其组成微生物在纤维素降解和其他重要代谢过程中相互协作。对纤维素分解种间合作的支持来自于发现F1RT微生物编码并表达互补的酶库，其中包括细胞外纤维小体和分泌型游离酶系统。对七种F1RT系统发育型的代谢重建预测了关于这个特定群落如何发挥功能的更广泛基因组原理，以及自然界中生物质转化为何依赖结构化和合作性微生物群落的可能原因。
