Sanjenbam P, Gopal J V, Kannabiran K
Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India.
Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India.
J Mycol Med. 2014 Sep;24(3):211-9. doi: 10.1016/j.mycmed.2014.03.004. Epub 2014 Jun 13.
The aim of the present study was to evaluate the anticandidal activity of biologically synthesized silver nanoparticles using the culture filtrate of Streptomyces sp.VITPK1.
Silver nanoparticles were synthesized using the culture filtrate of Streptomyces species isolated from brine spring located at Thoubal District, Manipur, India. The isolate was identified by molecular taxonomic characterization and designated as Streptomyces sp.VITPK1. The synthesized silver nanoparticles (AgNPs) were characterized by UV-visible spectra, X-ray diffraction (XRD) patterns, Energy Dispersive Analysis of X-rays (EDAX) and Fourier Transform Infrared (FTIR) analysis. The antifungal activity of the synthesized silver nanoparticles was evaluated against selected Candida species.
The synthesized AgNPs showed a surface plasmon resonance peak at 425 nm. XRD patterns showed the crystalline peaks at 38.15° (111), 44.35° (200), 64.52° (220) and 77.49° (311) matching with the diffraction facets of silver. The size of the AgNPs was in the range of 20-45 nm. The EDAX analysis revealed the presence of silver as the major metal in the sample. The synthesized AgNPs showed anticandidal activity against Candida albicans, Candida tropicalis and Candida krusei with a maximum zone of inhibition of 20mm against C. albicans.
The results of this study suggest that the green synthesis of silver nanoparticles using Streptomyces sp.VITPK1 have the ability to act against pathogenic Candida strains.
本研究旨在评估利用链霉菌属VITPK1的培养滤液生物合成的银纳米颗粒的抗念珠菌活性。
使用从印度曼尼普尔邦 Thoubal 区盐泉分离的链霉菌属的培养滤液合成银纳米颗粒。通过分子分类学表征鉴定该分离株，并将其命名为链霉菌属VITPK1。通过紫外可见光谱、X射线衍射（XRD）图谱、能量色散X射线分析（EDAX）和傅里叶变换红外（FTIR）分析对合成的银纳米颗粒（AgNPs）进行表征。评估合成的银纳米颗粒对选定念珠菌属的抗真菌活性。
合成的AgNPs在425nm处显示表面等离子体共振峰。XRD图谱显示在38.15°（111）、44.35°（200）、64.52°（220）和77.49°（311）处的结晶峰与银的衍射面匹配。AgNPs的尺寸在20 - 45nm范围内。EDAX分析表明样品中主要金属为银。合成的AgNPs对白色念珠菌、热带念珠菌和克柔念珠菌显示出抗念珠菌活性，对白色念珠菌的最大抑菌圈为20mm。
本研究结果表明，利用链霉菌属VITPK1绿色合成银纳米颗粒具有对抗致病性念珠菌菌株的能力。
