Srivastava Kumar Chandan, Austin Ravi David, Shrivastava Deepti, Pranavadhyani Gopalakrishnan
Department of Oral Medicine and Radiology, Rajah Muthiah Dental College and Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.
Department of Periodontics, Rajah Muthiah Dental College and Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.
Dent Res J (Isfahan). 2014 Mar;11(2):180-6.
Imbalances between the oxidant-antioxidant status have been implicated in the pathogenesis of several diseases, including oral cancer. Majority of oral cancer are preceded by a well-recognized group of pre-malignant lesions. However, only a small fraction of those lesions, undergo malignant transformation. Hence, there is a great need to identify biological markers, which will assist in identifying lesion carrying high-risk. This study aims to evaluate and compare the status of oxidative stress and antioxidant enzymes in tissue samples of patients with various clinicopathological stages of oral pre-malignancy.
A case control study consisting of 20 new histopathologically proven leukoplakia patients and equal number of age, sex, and habit matched healthy subjects were recruited for this study. Their tissue samples were subjected to evaluation of lipid peroxidation product, thiobarbituric acid reactive substances and antioxidant enzymes, namely, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and glutathione peroxidase (GPx) using spectrophotometric methods. The data are expressed as mean ± standard deviation. The statistical comparisons were performed by independent Student's unpaired t-test and one-way analysis of variance. Pearson's correlation was performed for the biochemical parameters within the group and between the groups. For statistically significant correlations, simple linear regression was performed. P- value < 0.05 was considered statistically significant.
Significant reduction in lipid peroxidation (P < 0.001) SOD and CAT (P < 0.001) was observed in the tissue of leukoplakia patients as compared to the healthy controls. On the other hand, GSH and GPx were significantly increased in tumor samples.
Reduced lipid peroxidation and increased activity of GSH and GPx provides the suitable environment for the tumor growth and malignant transformation in the later stages. Among the antioxidant enzymes, glutathione reductase appears to have a profound role in carcinogenesis.
氧化-抗氧化状态失衡与包括口腔癌在内的多种疾病的发病机制有关。大多数口腔癌之前都有一组公认的癌前病变。然而，这些病变中只有一小部分会发生恶性转化。因此，迫切需要识别生物标志物，以帮助识别具有高风险的病变。本研究旨在评估和比较口腔癌前病变不同临床病理阶段患者组织样本中的氧化应激和抗氧化酶状态。
本病例对照研究招募了20名新的经组织病理学证实的白斑患者以及数量相等、年龄、性别和习惯相匹配的健康受试者。使用分光光度法对他们的组织样本进行脂质过氧化产物、硫代巴比妥酸反应性物质以及抗氧化酶（即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx））的评估。数据以平均值±标准差表示。采用独立样本t检验和单因素方差分析进行统计学比较。对组内和组间的生化参数进行Pearson相关性分析。对于具有统计学意义的相关性，进行简单线性回归分析。P值<0.05被认为具有统计学意义。
与健康对照组相比，白斑患者组织中的脂质过氧化（P<0.001）、SOD和CAT（P<0.001）显著降低。另一方面，肿瘤样本中的GSH和GPx显著增加。
脂质过氧化减少以及GSH和GPx活性增加为肿瘤生长和后期恶性转化提供了适宜的环境。在抗氧化酶中，谷胱甘肽还原酶似乎在致癌过程中起重要作用。
