Reuk-ngam Nanthawan, Chimnoi Nitirat, Khunnawutmanotham Nisachon, Techasakul Supanna
Laboratory of Organic Synthesis, Chulabhorn Research Institute, Vibhavadi-Rangsit Highway, Laksi, Bangkok 10210, Thailand.
Laboratory of Natural Products, Chulabhorn Research Institute, Vibhavadi-Rangsit Highway, Laksi, Bangkok 10210, Thailand.
Biomed Res Int. 2014;2014:581985. doi: 10.1155/2014/581985. Epub 2014 May 15.
Coronarin D is a labdane-type diterpene from the rhizomes of Hedychium coronarium. In the view of our ongoing effort to explore its novel biological activity, antimicrobial activity study of coronarin D was performed. The results showed that coronarin D was active against tested Gram-positive bacteria, inactive for tested Gram-negative bacteria, and weakly active against tested fungi. The antibacterial effect of the combination of coronarin D with nine classical antibiotics against four Gram-positive bacteria was also evaluated. The fractional inhibitory concentration indices (FICI) of coronarin D-antibiotics combinations, calculated from the checkerboard assay, were used as synergism indicator. Out of 36 combinations, 47% showed total synergism, 33% had partial synergistic interaction, 17% showed no effect, and 3% showed antagonism. By combination with coronarin D at concentration of 0.25 minimal inhibitory concentration (MIC), the activities of antibiotics were boosted to 4- to 128-fold. These finding suggested an attractive approach to combat the infectious diseases by using coronarin D-antibiotic drug combination.
姜花素D是一种从姜花根茎中提取的半日花烷型二萜。鉴于我们正在努力探索其新的生物活性，对姜花素D进行了抗菌活性研究。结果表明，姜花素D对测试的革兰氏阳性菌有活性，对测试的革兰氏阴性菌无活性，对测试的真菌有微弱活性。还评估了姜花素D与九种经典抗生素联合对四种革兰氏阳性菌的抗菌效果。通过棋盘法计算的姜花素D -抗生素组合的部分抑菌浓度指数（FICI）用作协同作用指标。在36种组合中，47%表现出完全协同作用，33%有部分协同相互作用，17%无作用，3%表现出拮抗作用。通过与浓度为0.25最小抑菌浓度（MIC）的姜花素D联合，抗生素的活性提高了4至128倍。这些发现提示了一种通过使用姜花素D -抗生素药物组合来对抗传染病的有吸引力的方法。
