Yoon S Y, Eo S K, Kim Y S, Lee C K, Han S S
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Arch Pharm Res. 1994 Dec;17(6):438-42. doi: 10.1007/BF02979122.
Antimicrobial activity of GL (the aqueous extract from the carpophores of Ganoderma lucidum (FR)KARST) was tested in vitro against Gram positive and Gram negative bacteria by serial broth dilution method, and the antimicrobial activity was expressed by minimal inhibitory concentration (MIC). Among fifteen species of bacteria tested, the antimicrobial activity of GL was the most potent against Micrococcus luteus (MIC, 0.75 mg/ml). To investigate the effects of antimicrobial combinations of GL with four kinds of antibiotics (ampicillin, cefazolin, oxytetracycline and chloramphenicol), the fractional inhibitory concentration index (FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of GL with four antibiotics resulted in additive effect in most instances, synergism in two instances, and antagonism in two instances. Synergism was observed when GL was combined with cefazolin against Bacillus subtilis and Klebsiella oxytoca.
采用肉汤稀释法体外检测了灵芝子实体水提取物（GL）对革兰氏阳性菌和革兰氏阴性菌的抗菌活性，抗菌活性以最低抑菌浓度（MIC）表示。在所检测的15种细菌中，GL对藤黄微球菌的抗菌活性最强（MIC为0.75 mg/ml）。为研究GL与四种抗生素（氨苄青霉素、头孢唑林、土霉素和氯霉素）联合抗菌的效果，通过棋盘法测定了每种菌株的部分抑菌浓度指数（FICI）。GL与四种抗生素联合使用在大多数情况下产生相加作用，在两种情况下产生协同作用，在两种情况下产生拮抗作用。当GL与头孢唑林联合用于枯草芽孢杆菌和产酸克雷伯菌时观察到协同作用。
