Grup de Biotecnologia Molecular i Industrial, Department of Chemical Engineering, Universitat Politècnica de Catalunya , Rambla Sant Nebridi 22, 08222 Terrassa, Spain.
ACS Appl Mater Interfaces. 2014 Jul 23;6(14):11385-93. doi: 10.1021/am501961b. Epub 2014 Jul 3.
Catheter associated urinary tract infections are common during hospitalization due to the formation of bacterial biofilms on the indwelling device. In this study, we report an innovative biotechnology-based approach for the covalent functionalization of silicone catheters with antifouling zwitterionic moieties to prevent biofilm formation. Our approach combines the potential bioactivity of a natural phenolics layer biocatalytically conjugated to sulfobetaine-acrylic residues in an enzymatically initiated surface radical polymerization with laccase. To ensure sufficient coating stability in urine, the silicone catheter is plasma-activated. In contrast to industrial chemical methods, the methacrylate-containing zwitterionic monomers are polymerized at pH 5 and 50 °C using as an initiator the phenoxy radicals solely generated by laccase on the phenolics-coated catheter surface. The coated catheters are characterized by X-ray photoelectron spectroscopy (XPS), Fourier transformed infrared (FTIR) analysis, atomic force microscopy (AFM), and colorimetrically. Contact angle and protein adsorption measurements, coupled with in vitro tests with the Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus in static and dynamic conditions, mimicking the operational conditions to be faced by the catheters, demonstrate reduced biofilm formation by about 80% when compared to that of unmodified urinary catheters. The zwitterionic coating did not affect the viability of the human fibroblasts (BJ-5ta) over seven days, corresponding to the extended useful life of urinary catheters.
导管相关尿路感染在住院期间很常见，这是由于留置装置上形成了细菌生物膜。在本研究中，我们报告了一种基于创新生物技术的方法，通过共价功能化硅酮导管与抗污两性离子部分来防止生物膜形成。我们的方法结合了生物催化共轭到磺基甜菜碱 - 丙烯酰胺残基中的天然酚类层的潜在生物活性，以及漆酶引发的表面自由基聚合。为了确保在尿液中有足够的涂层稳定性，硅酮导管进行了等离子体激活。与工业化学方法相比，含有甲基丙烯酸酯的两性离子单体在 pH 5 和 50°C 下聚合，使用漆酶仅在酚类涂层导管表面上生成的苯氧自由基作为引发剂。通过 X 射线光电子能谱（XPS）、傅里叶变换红外（FTIR）分析、原子力显微镜（AFM）和比色法对涂层导管进行了表征。接触角和蛋白质吸附测量，以及在静态和动态条件下与革兰氏阴性铜绿假单胞菌和革兰氏阳性金黄色葡萄球菌的体外测试，模拟导管将面临的操作条件，表明与未改性的尿液导管相比，生物膜形成减少了约 80%。两性离子涂层在七天内没有影响人类成纤维细胞（BJ-5ta）的活力，这与尿液导管的延长使用寿命相对应。
