光热治疗金/硫化铜核壳纳米粒子对细菌孢子和细胞的抗菌活性研究。
Investigation of antimicrobial activity of photothermal therapeutic gold/copper sulfide core/shell nanoparticles to bacterial spores and cells.
机构信息
Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprises (BRITE), North Carolina Central University, Durham, NC 27707, USA.
Department of Physics, University of Texas at Arlington, Arlington, TX 76019, USA ; Guizhou Provincial Key Laboratory of Pharmaceutics, School of Pharmacy, Guiyang Medical College, Guiyang, Guizhou 550004, China.
出版信息
J Biol Eng. 2014 Jun 2;8:11. doi: 10.1186/1754-1611-8-11. eCollection 2014.
BACKGROUND
Au/CuS core/shell nanoparticles (NPs) were designed as a new type of transducer agent for photothermal therapy (PTT), with attractive features of easy preparation, low cost and small size for targeting. This paper studied for the first time the intrinsic antimicrobial activity of Au/CuS NPs to B. anthracis spores and cells in addition to its PTT effect.
RESULTS
It was found that Au/CuS NPs were highly efficient in inactivating B. anthracis cells, but not effective to the spores. Treatment with NPs at ~0.83 μM for 30 min achieved a 7 log reduction in viable cells. The antimicrobial effect was both NPs concentration and treatment time dependent. SEM imaging and the efflux of DNA test demonstrated the damage of cell membrane after NPs treatment, yet further research is necessary to fully understand the precise inactivation mechanism.
CONCLUSIONS
The Au/CuS NPs had strong antimicrobial activity to B. anthracis cells, which showed a great potential to be an effective antimicrobial agent to bacterial cells.
背景
Au/CuS 核/壳纳米粒子（NPs）被设计为光热治疗（PTT）的新型换能剂，具有易于制备、成本低和尺寸小等特点，可用于靶向治疗。本文首次研究了 Au/CuS NPs 对炭疽芽孢杆菌孢子和细胞的固有抗菌活性及其 PTT 效应。
结果
研究发现，Au/CuS NPs 能高效灭活炭疽杆菌细胞，但对孢子无效。在~0.83 μM 时处理 30 分钟，活菌数减少了 7 个对数级。抗菌效果与 NPs 浓度和处理时间有关。SEM 成像和 DNA 外排试验表明，NPs 处理后细胞膜受损，但要充分了解确切的失活机制，还需要进一步研究。
结论
Au/CuS NPs 对炭疽杆菌细胞具有很强的抗菌活性，有望成为一种有效的抗菌剂，用于细菌细胞。
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