Qin Hua, Brookes Philip C, Xu Jianming, Feng Youzhi
Institute of Soil and Water Resources and Environmental Science, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University, Hangzhou, 310058, China.
Environ Sci Pollut Res Int. 2014 Nov;21(22):12790-9. doi: 10.1007/s11356-014-3231-y. Epub 2014 Jun 28.
A greenhouse experiment was conducted to investigate the effects of zucchini (Cucurbita pepo L.), inoculated with the arbuscular mycorrhizal (AM) species Acaulospora laevis, Glomus caledonium, and Glomus mosseae, on the soil bacterial community responsible for Aroclor 1242 dissipation. The dissipation rates of Aroclor 1242 and soil bacteria abundance were much higher with the A. laevis and G. mosseae treatments compared to the non-mycorrhizal control. The biphenyl dioxygenase (bphA) and Rhodococcus-like 2,3-dihydroxybiphenyl dioxygenase (bphC) genes were more abundant in AM inoculated soils, suggesting that the bphA and Rhodococcus-like bphC pathways play an important role in Aroclor 1242 dissipation in the mycorrhizosphere. The soil bacterial communities were dominated by classes Betaproteobacteria and Actinobacteria, while the relative proportion of Actinobacteria was significantly (F=2.288, P<0.05) correlated with the PCB congener profile in bulk soil. Our results showed that AM fungi could enhance PCB dissipation by stimulating bph gene abundance and the growth of specific bacterial groups.
进行了一项温室试验，以研究接种丛枝菌根（AM）真菌光壁无梗囊霉、喀里多尼亚球囊霉和摩西球囊霉的西葫芦（南瓜属西葫芦种）对负责艾氏剂1242降解的土壤细菌群落的影响。与未接种菌根的对照相比，光壁无梗囊霉和摩西球囊霉处理下艾氏剂1242的降解率和土壤细菌丰度要高得多。在接种AM真菌的土壤中，联苯双加氧酶（bphA）和类红球菌2,3-二羟基联苯双加氧酶（bphC）基因更为丰富，这表明bphA和类红球菌bphC途径在菌根际艾氏剂1242的降解中起重要作用。土壤细菌群落以β-变形菌纲和放线菌纲为主，而放线菌纲的相对比例与土壤中多氯联苯同系物谱显著相关（F=2.288，P<0.05）。我们的结果表明，AM真菌可通过刺激bph基因丰度和特定细菌群体的生长来增强多氯联苯的降解。
