Pennell D R, Rott-Petri J A, Kurzynski T A
J Clin Microbiol. 1984 Oct;20(4):614-7. doi: 10.1128/jcm.20.4.614-617.1984.
Three commercially available rapid slide agglutination tests for the identification of Staphylococcus aureus were evaluated with 354 recent clinical isolates (165 strains of S. aureus). The test results of two latex agglutination products, SeroSTAT Staph (Scott Laboratories, Inc.) and Staphylatex (American Micro Scan), and one hemagglutination product, Staphyloslide (BBL Microbiology Systems), were compared with the results of the tube coagulase test, which was read at 4 h (4-h tube coagulase test) and, if negative, again after overnight incubation at room temperature (24-h tube coagulase test). Discrepancies between agglutination and tube coagulase identifications were resolved by use of the thermonuclease, mannitol fermentation, and slide coagulase tests. All sensitivities, specificities, predictive values of a positive result, and predictive values of a negative result for the three agglutination tests were at least 98.8% and comparable with the 4-h tube coagulase test. Best results were obtained with the 24-h tube coagulase test, which yielded one false-negative and no false-positive tests. Agglutination identifications may be performed on organisms taken directly from a primary plate when sufficient growth is present. Kit agglutination procedures yield rapid and reliable identifications and are easy to perform. This study also demonstrates the usefulness of the 24-h tube coagulase test.
采用三种市售快速玻片凝集试验对金黄色葡萄球菌进行鉴定，并对354株近期临床分离株（165株金黄色葡萄球菌）进行了评估。将两种乳胶凝集产品SeroSTAT Staph（斯科特实验室公司）和Staphylatex（美国微生物扫描公司）以及一种血凝产品Staphyloslide（BBL微生物系统公司）的检测结果与试管凝固酶试验结果进行比较，试管凝固酶试验在4小时读取结果（4小时试管凝固酶试验），如果结果为阴性，则在室温下过夜培养后再次读取（24小时试管凝固酶试验）。凝集试验和试管凝固酶鉴定之间的差异通过热核酸酶、甘露醇发酵和玻片凝固酶试验来解决。三种凝集试验的所有敏感性、特异性、阳性预测值和阴性预测值均至少为98.8%，与4小时试管凝固酶试验相当。24小时试管凝固酶试验结果最佳，该试验产生了1例假阴性结果，无假阳性结果。当有足够的生长物时，可对直接取自原始平板的微生物进行凝集鉴定。试剂盒凝集程序可快速、可靠地进行鉴定，且易于操作。本研究还证明了24小时试管凝固酶试验的实用性。
