National and Local Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Euro Surveill. 2014 Jun 26;19(25):20841. doi: 10.2807/1560-7917.es2014.19.25.20841.
Human infection with a novel influenza A(H10N8) virus was first described in China in December 2013. However, the origin and genetic diversity of this virus is still poorly understood. We performed a phylogenetic analysis and coalescent analysis of two viruses from the first case of influenza A(H10N8) (A/Jiangxi-Donghu/346-1/2013 and A/Jiangxi-Donghu/346-2/2013 and a novel A(H10N8) virus (A/chicken/Jiangxi/102/2013) isolated from a live poultry market that the patient had visited. The haemagglutinin (HA), neuraminidase (NA), PA subunit of the virus polymerase complex, nucleoprotein (NP), M and nonstructural protein (NS) genes of the three virus strains shared the same genetic origins. The origins of their HA and NA genes were similar: originally from wild birds to ducks, and then to chickens. The PA, NP, M, and NS genes were similar to those of chicken influenza A(H9N2) viruses. Coalescent analyses showed that the reassortment of these genes from A(H9N2) to A(H10N8) might have occurred at least twice. However, the PB1 and PB2 genes of the chicken A(H10N8) virus most likely originated from H7-like viruses of ducks, while those of the viruses from the case most likely stemmed from A(H9N2) viruses circulating in chickens. The oseltamivir-resistance mutation, R292K (R291K in A(H10N8) numbering) in the NA protein, occurred after four days of oseltamivir treatment. It seems that A(H10N8) viruses might have become established among poultry and their genetic diversity might be much higher than what we have observed.
人感染新型甲型流感病毒（H10N8）病毒于 2013 年 12 月在中国首次被描述。然而，该病毒的起源和遗传多样性仍不清楚。我们对来自首例甲型流感病毒（H10N8）（A/Jiangxi-Donghu/346-1/2013 和 A/Jiangxi-Donghu/346-2/2013）和新型 A（H10N8）病毒（A/chicken/Jiangxi/102/2013）的两个病毒进行了系统发育分析和合并分析，该病毒来自患者曾光顾的活禽市场。这三种病毒株的血凝素（HA）、神经氨酸酶（NA）、病毒聚合酶复合物的 PA 亚单位、核蛋白（NP）、M 和非结构蛋白（NS）基因具有相同的遗传起源。其 HA 和 NA 基因的起源相似：最初来源于野鸟到鸭，然后到鸡。PA、NP、M 和 NS 基因与鸡甲型流感（H9N2）病毒相似。合并分析表明，这些基因从 A（H9N2）到 A（H10N8）的重配至少发生了两次。然而，鸡 A（H10N8）病毒的 PB1 和 PB2 基因很可能源自鸭的 H7 样病毒，而病例中病毒的 PB1 和 PB2 基因很可能源自在鸡中流行的 A（H9N2）病毒。NA 蛋白中的奥司他韦耐药突变，R292K（A（H10N8）编号中的 R291K），发生在奥司他韦治疗四天后。似乎 A（H10N8）病毒已经在禽类中建立起来，其遗传多样性可能比我们观察到的要高得多。
