Jia Ru, Lu Yi, Yang Chang-Wei, Luo Xiao, Han Ying
Department of Prosthodontics, Stomatological Hospital, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China; Department of Physiology and Pathophysiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.
Department of Prosthodontics, Stomatological Hospital, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Arch Oral Biol. 2014 Oct;59(10):1085-93. doi: 10.1016/j.archoralbio.2014.06.008. Epub 2014 Jun 24.
Dentine hypersensitivity is a type of clinical oral disease, which is highly prevalent worldwide. Although there are many materials to treat dentine hypersensitivity, their long-term therapeutic effects are not satisfactory. Therefore, the aim of this research was to observe and identify the biological mineralization of the generation 4.0 polyamidoamine dendrimer on the demineralized dentinal tubules at different time points.
2mm-thick slices were obtained from the cemento-enamel junction of 36 third molar teeth that simulated the condition of sensitivity with acid etching. Slices were treated with generation 4.0 polyamidoamine dendrimer and peptide bond condensing agent, while no treatment was applied on the slices of the control group. Following immersion in artificial saliva for 2, 4, 6, and 8 weeks respectively, the mineralization condition of dentine slices was observed using the scanning electron microscope (SEM). In addition, the differences in the samples of dental slices between the 2 groups were also detected using the microhardness test.
SEM results showed that the average diameter and density of the dentinal tubules in the experimental group were significantly lower than those in the control group (P<0.001). The microhardness test exhibited a similar result, which suggested that the microhardness of the experimental group was significantly higher than the control group (P<0.001).
Generation 4.0 polyamidoamine dendrimer promotes the biomineralization of demineralized dentinal tubules. Moreover, this result also suggests that the 4.0th generation polyamidoamine dendrimer has the potential value for dentine hypersensitivity treatment.
牙本质过敏症是一种临床口腔疾病，在全球范围内高度流行。尽管有许多材料可用于治疗牙本质过敏症，但其长期治疗效果并不理想。因此，本研究的目的是观察并确定第4.0代聚酰胺-胺树枝状大分子在不同时间点对脱矿牙本质小管的生物矿化作用。
从36颗第三磨牙的牙骨质-釉质界处获取2毫米厚的切片，用酸蚀法模拟敏感状态。将切片用第4.0代聚酰胺-胺树枝状大分子和肽键缩合剂处理，而对照组的切片不做处理。分别在人工唾液中浸泡2、4、6和8周后，使用扫描电子显微镜（SEM）观察牙本质切片的矿化情况。此外，还使用显微硬度测试检测两组牙片样本之间的差异。
SEM结果显示，实验组牙本质小管的平均直径和密度显著低于对照组（P<0.001）。显微硬度测试也呈现出类似结果，表明实验组的显微硬度显著高于对照组（P<0.001）。
第4.0代聚酰胺-胺树枝状大分子促进脱矿牙本质小管的生物矿化。此外，该结果还表明第4.0代聚酰胺-胺树枝状大分子在治疗牙本质过敏症方面具有潜在价值。
