Popovich Phillip G, Tovar C Amy, Lemeshow Stanley, Yin Qin, Jakeman Lyn B
Center for Brain and Spinal Cord Repair, USA; Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH, USA.
Center for Brain and Spinal Cord Repair, USA; Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH, USA.
Exp Neurol. 2014 Nov;261:97-108. doi: 10.1016/j.expneurol.2014.06.020. Epub 2014 Jul 3.
The goal of the current manuscript was to replicate published data that show intrathecal infusions of Taxol® (paclitaxel), an anti-neoplastic microtubule stabilizing agent, reduce fibrogliotic scarring caused by a dorsal spinal hemisection (DHx) injury and increase functional recovery and growth of serotonergic axons after moderate spinal contusion injury. These experiments were completed as part of an NIH-NINDS contract entitled "Facilities of Research Excellence in Spinal Cord Injury (FORE-SCI) - Replication". Here, data are presented that confirm the anti-scarring effects of Taxol after DHx injury; however, Taxol did not confer neuroprotection or promote serotonergic axon growth nor did it improve functional recovery in a model of moderate spinal contusion injury. Thus, only partial replication was achieved. Possible explanations for disparate results in our studies and published data are discussed.
本手稿的目的是复制已发表的数据，这些数据表明鞘内注射抗肿瘤微管稳定剂紫杉醇（Taxol®）可减少脊髓背侧半横断（DHx）损伤引起的纤维性瘢痕形成，并在中度脊髓挫伤损伤后增加5-羟色胺能轴突的功能恢复和生长。这些实验是作为美国国立卫生研究院（NIH）-国立神经疾病和中风研究所（NINDS）名为“脊髓损伤卓越研究设施（FORE-SCI）-复制”合同的一部分完成的。在此，我们展示的数据证实了紫杉醇在DHx损伤后的抗瘢痕作用；然而，在中度脊髓挫伤损伤模型中，紫杉醇并未提供神经保护作用，也未促进5-羟色胺能轴突生长，也未改善功能恢复。因此，仅实现了部分复制。我们讨论了我们的研究结果与已发表数据存在差异的可能原因。
