Liu Shuo, Zhao Yue, Parks Joshua W, Deamer David W, Hawkins Aaron R, Schmidt Holger
School of Engineering, University of California, Santa Cruz , 1156 High Street, Santa Cruz, California 95064, United States.
Nano Lett. 2014 Aug 13;14(8):4816-20. doi: 10.1021/nl502400x. Epub 2014 Jul 11.
The analysis of individual biological nanoparticles has significantly advanced our understanding of fundamental biological processes but is also rapidly becoming relevant for molecular diagnostic applications in the emerging field of personalized medicine. Both optical and electrical methods for the detection and analysis of single biomolecules have been developed, but they are generally not used in concert and in suitably integrated form to allow for multimodal analysis with high throughput. Here we report on a dual-mode electrical and optical single-nanoparticle sensing device with capabilities that would not be available with each technique individually. The new method is based on an optofluidic chip with an integrated nanopore that serves as a smart gate to control the delivery of individual nanoparticles to an optical excitation region for ensemble-free optical analysis in rapid succession. We demonstrate electro-optofluidic size discrimination of fluorescent nanobeads, electro-optical detection of single fluorescently labeled influenza viruses, and the identification of single viruses within a mixture of equally sized fluorescent nanoparticles with up to 100% fidelity.
对单个生物纳米颗粒的分析极大地推动了我们对基本生物过程的理解，而且在个性化医疗这个新兴领域中，它对于分子诊断应用也正迅速变得至关重要。用于检测和分析单个生物分子的光学和电学方法都已得到发展，但它们通常并未协同使用，也没有以适当的集成形式来实现高通量的多模态分析。在此，我们报道了一种双模式电学和光学单纳米颗粒传感装置，其具备的功能是每种技术单独使用时所无法实现的。这种新方法基于一种带有集成纳米孔的光流体芯片，该纳米孔充当一个智能门控，用于控制单个纳米颗粒递送至光学激发区域，以便快速连续地进行无集合光学分析。我们展示了对荧光纳米珠的电光流体尺寸辨别、对单个荧光标记流感病毒的电光检测，以及在尺寸相同的荧光纳米颗粒混合物中以高达100%的保真度识别单个病毒。
