Pietropaoli Davide, Del Pinto Rita, Corridoni Daniele, Rodriguez-Palacios Alexander, Di Stefano Gabriella, Monaco Annalisa, Weinberg Aaron, Cominelli Fabio
Department of Medicine and Pathology, and Digestive Health Research Center, Case Western Reserve University, Cleveland, OH.
J Periodontol. 2014 Dec;85(12):1799-805. doi: 10.1902/jop.2014.140316.
Oral involvement is often associated with inflammatory bowel disease (IBD). Recent evidence suggests a high incidence of periodontal disease in patients with Crohn disease (CD). To the best of the authors' knowledge, no animal model of IBD that displays associated periodontal disease was reported previously. The aim of this study is to investigate the occurrence and progression of periodontal disease in SAMP1/YitFc (SAMP) mice that spontaneously develop a CD-like ileitis. In addition, the temporal correlation between the onset and progression of periodontal disease and the onset of ileitis in SAMP mice was studied.
At different time points, SAMP and parental AKR/J (AKR) control mice were sacrificed, and mandibles were prepared for stereomicroscopy and histology. Terminal ilea were collected for histologic assessment of inflammation score. Periodontal status, i.e., alveolar bone loss (ABL) and alveolar bone crest, was examined by stereomicroscopy and histomorphometry, respectively.
ABL increased in both strains with age. SAMP mice showed greater ABL compared with AKR mice by 12 weeks of age, with maximal differences observed at 27 weeks of age. AKR control mice did not show the same severity of periodontal disease. Interestingly, a strong positive correlation was found between ileitis severity and ABL in SAMP mice, independent of age.
The present results demonstrate the occurrence of periodontal disease in a mouse model of progressive CD-like ileitis. In addition, the severity of periodontitis strongly correlated with the severity of ileitis, independent of age, suggesting that common pathogenic mechanisms, such as abnormal immune response and dysbiosis, may be shared between these two phenotypes.
口腔受累常与炎症性肠病（IBD）相关。最近的证据表明，克罗恩病（CD）患者中牙周病的发病率很高。据作者所知，此前尚未报道过表现出相关牙周病的IBD动物模型。本研究的目的是调查自发发生类CD回肠炎的SAMP1/YitFc（SAMP）小鼠中牙周病的发生和进展情况。此外，还研究了SAMP小鼠牙周病的发病和进展与回肠炎发病之间的时间相关性。
在不同时间点处死SAMP小鼠和作为亲本的AKR/J（AKR）对照小鼠，并制备下颌骨用于立体显微镜检查和组织学检查。收集末端回肠进行炎症评分的组织学评估。分别通过立体显微镜检查和组织形态计量学检查牙周状况，即牙槽骨吸收（ABL）和牙槽嵴。
两种品系的小鼠ABL均随年龄增加。到12周龄时，SAMP小鼠的ABL比AKR小鼠更大，在27周龄时观察到最大差异。AKR对照小鼠未表现出相同严重程度的牙周病。有趣的是，在SAMP小鼠中，回肠炎严重程度与ABL之间发现了强正相关，且与年龄无关。
目前的结果证明了在进行性类CD回肠炎小鼠模型中存在牙周病。此外，牙周炎的严重程度与回肠炎的严重程度密切相关，且与年龄无关，这表明这两种表型之间可能存在共同的致病机制，如异常免疫反应和微生物群落失调。
