环（l-脯氨酰-l-亮氨酰）分离自南极土壤的 MCCC 1A00316：对. 的活性鉴定和表征
Cyclo(l-Pro⁻l-Leu) of MCCC 1A00316 Isolated from Antarctic Soil: Identification and Characterization of Activity against .
机构信息
State Key Laboratory of Agricultural Microbiology and National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China.
出版信息
Molecules. 2019 Feb 20;24(4):768. doi: 10.3390/molecules24040768.
MCCC 1A00316 was originally isolated from an Antarctic soil and has demonstrated potential nematicidal activity. Thus, it has promising applications for the biological control of . The larval mortality and egg-hatching inhibition rates of will increase with the rising concentration of culture filtrates of MCCC 1A00316 and the duration of exposure. Thus, this study aimed to separate, purify, and identify nematicidal compounds from MCCC 1A00316 and to validate their anti- activities. Compounds were purified through silica gel column chromatography and thin-layer chromatography combined with high-performance liquid chromatography (HPLC). Structural identification was conducted through liquid chromatography time-of-flight mass spectrometry, ¹H nuclear magnetic resonance (NMR) spectroscopy, C-NMR, and Marfey's method. The isolated compounds were identified as cyclo(l-Pro⁻l-Leu) on the basis of the results of the above analyses and previously reported data. The effects of various concentrations of cyclo(l-Pro⁻l-Leu) on the mortality rates of second-stage juveniles (J2) of were investigated. Results showed that HPLC-purified cyclo(l-Pro⁻l-Leu) displayed nematicidal activities. The mortality rate of J2 reached 84.3% after 72 h of exposure to 67.5 mg/L cyclo(l-Pro⁻l-Leu). The lowest egg-hatching rate (9.74%) was observed after 8 days of incubation with 2000 mg/L cyclo(l-Pro⁻l-Leu). An egg-hatching rate of 53.11% was obtained under the control treatment (sterile distilled water). However, cyclo(l-Pro⁻l-Leu) did not elicit chemotaxis activity to . This is the first work to investigate the anti- characteristics of cyclo(l-Pro⁻l-Leu).
MCCC 1A00316 最初从南极土壤中分离出来，具有潜在的杀线虫活性。因此，它在 的生物防治中有很好的应用前景。随着 MCCC 1A00316 培养液浓度和暴露时间的增加， 的幼虫死亡率和卵孵化抑制率也会增加。因此，本研究旨在从 MCCC 1A00316 中分离、纯化和鉴定杀线虫化合物，并验证其抗 的活性。化合物通过硅胶柱色谱和薄层层析结合高效液相色谱（HPLC）进行分离和纯化。通过液质联用、1H 核磁共振（NMR）波谱、C-NMR 和 Marfey 法进行结构鉴定。根据上述分析和以前的数据报告，分离得到的化合物被鉴定为环（l-Pro-l-Leu）。研究了不同浓度的环（l-Pro-l-Leu）对 2 龄幼虫（J2）死亡率的影响。结果表明，HPLC 纯化的环（l-Pro-l-Leu）具有杀线虫活性。暴露于 67.5 mg/L 环（l-Pro-l-Leu）72 h 后，J2 的死亡率达到 84.3%。用 2000 mg/L 环（l-Pro-l-Leu）孵育 8 天后，观察到最低的卵孵化率（9.74%）。对照组（无菌蒸馏水）的卵孵化率为 53.11%。然而，环（l-Pro-l-Leu）对 没有趋化活性。这是首次研究环（l-Pro-l-Leu）的抗 特性。
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