Tahara Tomomitsu, Shibata Tomoyuki, Kawamura Tomohiko, Okubo Masaaki, Ichikawa Yuichiro, Sumi Kazuya, Miyata Masahiro, Ishizuka Takamitsu, Nakamura Masakatsu, Nagasaka Mitsuo, Nakagawa Yoshihito, Ohmiya Naoki, Arisawa Tomiyasu, Hirata Ichiro
Department of Gastroenterology, Fujita Health University School of Medicine, 1-98 Dengakugakubo Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan,
Dig Dis Sci. 2015 Jan;60(1):205-10. doi: 10.1007/s10620-014-3316-y. Epub 2014 Aug 8.
Fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. Here, we performed prevalence screening of Fusobacterium in ulcerative colitis (UC) in Japanese patients.
We examined Fusobacterium nucleatum (F. nucleatum) and whole Fusobacterium species (Pan-fusobacterium) by quantitative real-time PCR in 163 inflamed mucosae from 152 UC patients. Data were correlated with clinical subtypes of UC.
In an initial prevalence screen, F. nucleatum and Pan-fusobacterium were detected in 6.3 % (4/64) and 53.1 % (34/64). For all 163 mucosae, the prevalence of Pan-fusobacterium was 54.6 % (89/163). Pan-fusobacterium status was concordant in inflamed and normal adjacent samples, and the matched cases during 1-year follow-up colonoscopy. The higher amount of Pan-fusobacterium was observed in chronic continuous type compared to one attack and relapse/remitting type (p = 0.039). The higher amount of Pan-fusobacterium was also associated with rather mild clinical course of disease, such as non-steroid dependency (p = 0.015), non-refractory phenotype (p = 0.013), and non-severe phenotype (p = 0.04). Based on the distribution of Pan-fusobacterium measurable cases, we identified 10 cases as having a high amount of Pan-fusobacterium (FB-high). The clinicopathological features of FB-high UC cases were also highlighted by chronic continuous type and mild phenotypes of disease.
Whole Fusobacterium species, but not F. nucleatum, are common in UC patients and have a role in persistence of colonic inflammation in UC. However, Fusobacterium infection is associated with rather mild clinical phenotypes of UC.
梭杆菌属是人类肠道微生物群的一部分，但一些菌种已被确认为机会性病原体，与包括炎症性肠病在内的炎症性疾病有关。在此，我们对日本溃疡性结肠炎（UC）患者中的梭杆菌进行了患病率筛查。
我们通过定量实时PCR检测了152例UC患者163份炎症黏膜中的具核梭杆菌（F. nucleatum）和全梭杆菌属（泛梭杆菌）。数据与UC的临床亚型相关。
在初步患病率筛查中，具核梭杆菌和泛梭杆菌的检出率分别为6.3%（4/64）和53.1%（34/64）。在所有163份黏膜样本中，泛梭杆菌的患病率为54.6%（89/163）。炎症样本与相邻正常样本以及1年随访结肠镜检查中的匹配病例中，泛梭杆菌状态一致。与单次发作型和复发/缓解型相比，慢性持续型中泛梭杆菌数量更高（p = 0.039）。泛梭杆菌数量较高还与疾病相对较轻的临床病程相关，如非类固醇依赖（p = 0.015）、非难治性表型（p = 0.013）和非严重表型（p = 0.04）。根据可测量泛梭杆菌病例的分布，我们确定了10例泛梭杆菌数量高的病例（FB高）。FB高的UC病例的临床病理特征也以慢性持续型和疾病的轻度表型为突出特点。
全梭杆菌属而非具核梭杆菌在UC患者中很常见，并且在UC结肠炎症的持续存在中起作用。然而，梭杆菌感染与UC相对较轻的临床表型相关。
