Ishikawa Sayano, Hashizume Kouhei, Nishigori Hideo, Tezuka Yu, Sanbe Atsushi, Kurosaka Daijiro
Department of Ophthalmology, School of Medicine, Iwate Medical University , Morioka , Japan and.
Curr Eye Res. 2015 May;40(5):535-40. doi: 10.3109/02713683.2014.935445. Epub 2014 Aug 11.
To examine whether astaxanthin (AST) prevent the cataract formation induced by glucocorticoid in chick embryo.
Hydrocortisone hemisuccinate sodium (HC) (0.5 μmol/egg) was administered directly into the air chamber in the egg shell of chick embryo day 15. The eggs were then kept in an incubator at same conditions and administered 100 μL of 50 (HC + AST50 group), 80 (HC + AST80 group), 100 (HC + AST100 group) mg/mL of AST solutions dissolved in dimethyl sulfoxide (DMSO) 3 h after administration of HC. In addition, non-HC treated group (treated with physiological saline without HC and 100 μL of DMSO), HC-alone group (treated with 0.5 μmol of HC and 100 μL of DMSO), and AST100 group (treated with physiological saline without HC and 100 μL of DMSO) were also incorporated. After 48 h of treatment, lenses were removed from embryo and classified into five stages according to developed opacity. The amounts of reduced glutathione in the lenses and the blood glucose levels were measured.
The average scores of lens opacitiy were 2.63 ± 1.02 nmol/lens (HC-alone), 2.78 ± 0.97 nmol/lens (HC + AST50), 2.22 ± 1.20 nmol/lens (HC + AST80) and 1.84 ± 0.83 nmol/lens (HC + AST100; p < 0.05), respectively. Administration of AST decreased the lens opacity dose-dependently. The amounts of reduced glutathione in lenses were 11.6 ± 2.8 nmol/lens (HC-alone), 11.3 ± 2.7 nmol/lens (HC + AST50), 13.4 ± 2.4 nmol/lens (HC + AST80) and 13.7 ± 3.1 nmol/lens (HC + AST100; p < 0.05), respectively. Higher levels of AST prevented loss of reduced glutathione from the lens.
These findings support that AST protects glucocorticoid-induced cataract in chick embryo.
研究虾青素（AST）是否能预防糖皮质激素诱导的鸡胚白内障形成。
在鸡胚第15天，将半琥珀酸氢化可的松钠（HC）（0.5 μmol/枚鸡蛋）直接注入蛋壳内的气室。然后将鸡蛋置于相同条件的孵化器中，在注射HC 3小时后，分别给鸡蛋注射100 μL浓度为50（HC + AST50组）、80（HC + AST80组）、100（HC + AST100组）mg/mL的溶解于二甲基亚砜（DMSO）的AST溶液。此外，还设置了未用HC处理组（用不含HC的生理盐水和100 μL DMSO处理）、单独使用HC组（用0.5 μmol HC和100 μL DMSO处理）以及AST100组（用不含HC的生理盐水和100 μL DMSO处理）。处理48小时后，从胚胎中取出晶状体，并根据晶状体混浊程度分为五个阶段。测量晶状体中还原型谷胱甘肽的含量和血糖水平。
晶状体混浊平均评分分别为2.63±1.02 nmol/晶状体（单独使用HC组）、2.78±0.97 nmol/晶状体（HC + AST50组）、2.22±1.20 nmol/晶状体（HC + AST80组）和1.84±0.83 nmol/晶状体（HC + AST100组；p < 0.05）。AST的给药呈剂量依赖性地降低晶状体混浊程度。晶状体中还原型谷胱甘肽的含量分别为11.6±2.8 nmol/晶状体（单独使用HC组）、11.3±2.7 nmol/晶状体（HC + AST50组）、13.4±2.4 nmol/晶状体（HC + AST80组）和13.7±3.1 nmol/晶状体（HC + AST100组；p < 0.05）。较高剂量的AST可防止晶状体中还原型谷胱甘肽的丢失。
这些研究结果支持AST可保护鸡胚免受糖皮质激素诱导的白内障。
