Lebendiker Mario, Danieli Tsafi, de Marco Ario
Department of Biomedical Sciences and Engineering, University of Nova Gorica, Glavni Trg 9, SI-5261 Vipava, Slovenia.
BMC Res Notes. 2014 Sep 1;7:585. doi: 10.1186/1756-0500-7-585.
In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is extremely important on one hand to critically evaluate the quality of the proteins used in the described research and, on the other hand, to allow other laboratories to safely use the described procedure in a reproducible manner. We herby propose to include a standardized methodology that can easily be incorporated in research papers. Moreover, this methodology can be utilized as a "quality control" ladder, where the more information given, will lead to a higher ranking of the article. This "quality control" stamp will allow researchers retrieving relevant and useful materials and methods in the field of protein research.
在许多研究文章中，当各种分析（蛋白质-蛋白质相互作用、活性分析等）需要蛋白质纯化时，我们总是能看到最终结果，但很少能获取准确评估蛋白质质量所需的原始数据。这些数据一方面对于严格评估所描述研究中使用的蛋白质质量极为重要，另一方面能让其他实验室以可重复的方式安全地使用所描述的方法。我们特此提议纳入一种可轻松融入研究论文的标准化方法。此外，这种方法可用作一个“质量控制”阶梯，提供的信息越多，文章的排名就越高。这种“质量控制”标识将使研究人员能够检索蛋白质研究领域相关且有用的材料和方法。
