Occupational Lung Diseases Institute, Korea Workers' Compensation and Welfare Service, Ansan, Korea.
Saf Health Work. 2014 Jun;5(2):91-6. doi: 10.1016/j.shaw.2014.03.001. Epub 2014 Apr 2.
Chronic obstructive pulmonary disease (COPD) is an important cause of occupational mortality in miners exposed to coal mine dust. Although the inflammatory mediators involved in COPD have not been defined, many studies have shown that inflammatory mediators such as reactive oxygen and nitrogen species are involved in orchestrating the complex inflammatory process in COPD.
To investigate the relevance of exhaled biomarkers of oxidative and nitrosative stress in participants with COPD, we determined the levels of hydrogen peroxide, malondialdehyde (MDA), and 3-nitrotyrosine (3-NT) in exhaled breath condensate (EBC) in 90 retired elderly coal miners (53 non-COPD and 37 COPD participants).
Mean levels of MDA (4.64 nM vs. 6.46 nM, p = 0.005) and 3-NT (3.51 nM vs. 5.50 nM, p = 0.039) in EBC were significantly higher in participants with COPD. The median level of MDA did show statistical difference among the COPD severities (p = 0.017), and the area under the receiver operating characteristic curve for MDA (0.67) for the diagnostic discrimination of COPD indicated the biomarker. The optimal cutoff values were 5.34 nM (64.9% sensitivity and 64.2% specificity) and 5.58 nM (62.2% sensitivity and 62.3% specificity) for MDA and 3-NT, respectively. The results suggest that high levels of MDA and 3-NT in EBC are associated with COPD in retired elderly miners.
These results showed that the elevated levels of EBC MDA and EBC 3-NT in individuals with COPD are biomarkers of oxidative or nitrosative stress.
慢性阻塞性肺疾病（COPD）是暴露于煤矿粉尘的矿工职业性死亡的重要原因。虽然 COPD 涉及的炎症介质尚未确定，但许多研究表明，活性氧和氮物种等炎症介质参与调节 COPD 中的复杂炎症过程。
为了研究 COPD 患者呼出气氧化应激和硝化应激生物标志物的相关性，我们测定了 90 名退休老年矿工（53 名非 COPD 患者和 37 名 COPD 患者）呼出气冷凝物（EBC）中过氧化氢、丙二醛（MDA）和 3-硝基酪氨酸（3-NT）的水平。
EBC 中 MDA（4.64 nM 对 6.46 nM，p=0.005）和 3-NT（3.51 nM 对 5.50 nM，p=0.039）的平均水平在 COPD 患者中明显更高。MDA 的中位数水平在 COPD 严重程度之间存在统计学差异（p=0.017），MDA 的受试者工作特征曲线下面积（0.67）表明该生物标志物可用于 COPD 的诊断鉴别。MDA 和 3-NT 的最佳截断值分别为 5.34 nM（64.9%的敏感性和 64.2%的特异性）和 5.58 nM（62.2%的敏感性和 62.3%的特异性）。结果表明，EBC 中 MDA 和 3-NT 的高水平与退休老年矿工的 COPD 有关。
这些结果表明，COPD 个体 EBC MDA 和 EBC 3-NT 水平升高是氧化或硝化应激的生物标志物。
