Parham Kourosh, Sacks Daniel, Bixby Catherine, Fall Pamela
Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of Connecticut Health Center, Farmington, Connecticut, USA
Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of Connecticut Health Center, Farmington, Connecticut, USA.
Otolaryngol Head Neck Surg. 2014 Dec;151(6):1038-40. doi: 10.1177/0194599814551127. Epub 2014 Sep 22.
Serum biomarkers detect the earliest events in disease, monitor management, and provide insight into disease pathogenesis. At this time, there are no biomarkers available for otologic disorders. Otolin-1 is a scaffolding protein exclusively expressed in otoconia and cells of the vestibule and the cochlea; therefore, it may be a biomarker candidate for assessing the health of the inner ear. As a proof of concept, we used serum samples from controls without otologic history and subjects with a history of benign paroxysmal positional vertigo (BPPV), performed enzyme-linked immunosorbent assay for otolin-1, and measured the optical density of the substrate. Otolin-1 was detectable and quantifiable in all subjects, indicating that this inner ear protein crosses the blood-labyrinthine barrier. Furthermore, subjects with BPPV had significantly higher levels, with about one-third being above the control range. This promising preliminary result suggests that inner ear-specific proteins have the potential to serve as biomarkers for otologic disease processes.
血清生物标志物可检测疾病的最早事件、监测治疗情况并深入了解疾病发病机制。目前，尚无用于耳科疾病的生物标志物。耳石素-1是一种支架蛋白，仅在前庭和耳蜗的耳石及细胞中表达；因此，它可能是评估内耳健康的生物标志物候选物。作为概念验证，我们使用了无耳科病史的对照组和有良性阵发性位置性眩晕（BPPV）病史的受试者的血清样本，进行了耳石素-1的酶联免疫吸附测定，并测量了底物的光密度。在所有受试者中均可检测到并定量耳石素-1，表明这种内耳蛋白可穿过血迷路屏障。此外，BPPV受试者的水平显著更高，约三分之一高于对照范围。这一有前景的初步结果表明，内耳特异性蛋白有潜力作为耳科疾病过程的生物标志物。
