Ventre Maurizio, Natale Carlo Fortunato, Rianna Carmela, Netti Paolo Antonio
Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Piazzale Tecchio 80, 80125 Naples, Italy Interdisciplinary Research Center on Biomaterials, University of Naples Federico II, Piazzale Tecchio 80, 80125 Naples, Italy Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy.
Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy.
J R Soc Interface. 2014 Nov 6;11(100):20140687. doi: 10.1098/rsif.2014.0687.
Topographic patterns are known to affect cellular processes such as adhesion, migration and differentiation. However, the optimal way to deliver topographic signals to provide cells with precise instructions has not been defined yet. In this work, we hypothesize that topographic patterns may be able to control the sensing and adhesion machinery of cells when their interval features are tuned on the characteristic lengths of filopodial probing and focal adhesions (FAs). Features separated by distance beyond the length of filopodia cannot be readily perceived; therefore, the formation of new adhesions is discouraged. If, however, topographic features are separated by a distance within the reach of filopodia extension, cells can establish contact between adjacent topographic islands. In the latter case, cell adhesion and polarization rely upon the growth of FAs occurring on a specific length scale that depends on the chemical properties of the surface. Topographic patterns and chemical properties may interfere with the growth of FAs, thus making adhesions unstable. To test this hypothesis, we fabricated different micropatterned surfaces displaying feature dimensions and adhesive properties able to interfere with the filopodial sensing and the adhesion maturation, selectively. Our data demonstrate that it is possible to exert a potent control on cell adhesion, elongation and migration by tuning topographic features' dimensions and surface chemistry.
已知拓扑结构模式会影响细胞过程，如黏附、迁移和分化。然而，尚未确定向细胞传递拓扑信号以提供精确指令的最佳方式。在这项工作中，我们假设当拓扑结构模式的间隔特征在丝状伪足探测和粘着斑（FAs）的特征长度上进行调整时，它们可能能够控制细胞的传感和黏附机制。间隔距离超过丝状伪足长度的特征不容易被感知；因此，新黏附的形成受到抑制。然而，如果拓扑特征之间的距离在丝状伪足延伸范围内，细胞可以在相邻的拓扑岛之间建立接触。在后一种情况下，细胞黏附和极化依赖于在特定长度尺度上发生的粘着斑的生长，该长度尺度取决于表面的化学性质。拓扑结构模式和化学性质可能会干扰粘着斑的生长，从而使黏附不稳定。为了验证这一假设，我们制备了不同的微图案化表面，这些表面的特征尺寸和黏附特性能够选择性地干扰丝状伪足传感和黏附成熟。我们的数据表明，通过调整拓扑特征的尺寸和表面化学性质，可以对细胞黏附、伸长和迁移进行有效控制。
