Department of Prosthetic Dentistry, Center for Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106, Freiburg, Germany.
Department of Oral Biotechnology, Center for Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106, Freiburg, Germany.
Sci Rep. 2021 Aug 27;11(1):17302. doi: 10.1038/s41598-021-96526-x.
Plasma-treatment of oral implant biomaterials prior to clinical insertion is envisaged as a potential surface modification method for enhanced implant healing. To investigate a putative effect of plasma-functionalized implant biomaterials on oral tissue cells, this investigation examined the response of alveolar bone osteoblasts and gingival fibroblasts to clinically established zirconia- and titanium-based implant surfaces for bone and soft tissue integration. The biomaterials were either functionalized with oxygen-plasma in a plasma-cleaner or left untreated as controls, and were characterized in terms of topography and wettability. For the biological evaluation, the cell adhesion, morphogenesis, metabolic activity and proliferation were examined, since these parameters are closely interconnected during cell-biomaterial interaction. The results revealed that plasma-functionalization increased implant surface wettability. The magnitude of this effect thereby depended on surface topography parameters and initial wettability of the biomaterials. Concerning the cell response, plasma-functionalization of smooth surfaces affected initial fibroblast morphogenesis, whereas osteoblast morphology on rough surfaces was mainly influenced by topography. The plasma- and topography-induced differential cell morphologies were however not strong enough to trigger a change in proliferation behaviour. Hence, the results indicate that oxygen plasma-functionalization represents a possible cytocompatible implant surface modification method which can be applied for tailoring implant surface wettability.
在临床插入之前对口腔种植体生物材料进行等离子体处理被认为是一种增强种植体愈合的潜在表面改性方法。为了研究等离子体功能化种植体生物材料对口腔组织细胞的潜在影响，本研究检测了骨和软组织整合用临床确立的氧化锆和钛基种植体表面对牙槽骨成骨细胞和牙龈成纤维细胞的反应。生物材料要么在等离子清洗器中用氧等离子体进行功能化，要么作为对照不进行处理，并根据形貌和润湿性进行了表征。对于生物评估，检查了细胞黏附、形态发生、代谢活性和增殖，因为这些参数在细胞-生物材料相互作用过程中是密切相关的。结果表明，等离子体功能化增加了种植体表面的润湿性。这种效果的大小取决于表面形貌参数和生物材料的初始润湿性。关于细胞反应，光滑表面的等离子体功能化影响初始成纤维细胞形态发生，而粗糙表面上成骨细胞的形态主要受形貌影响。然而，等离子体和形貌引起的细胞形态差异还不足以引起增殖行为的改变。因此，结果表明，氧等离子体功能化代表了一种可能的细胞相容的种植体表面改性方法，可用于调整种植体表面的润湿性。
