Dahabreh Ziad, Panteli Michalis, Pountos Ippokratis, Howard Mark, Campbell Peter, Giannoudis Peter V
Ziad Dahabreh, Michalis Panteli, Ippokratis Pountos, Peter V Giannoudis, Academic Department of Trauma and Orthopaedics, School of Medicine, University of Leeds, LS1 3EX Leeds, United Kingdom.
World J Stem Cells. 2014 Sep 26;6(4):497-504. doi: 10.4252/wjsc.v6.i4.497.
To compare seven commercially available bone graft substitutes (BGS) in terms of these properties and without using any additional biological growth factors.
Porcine osteoprogenitor cells were loaded on seven commercially available BGS and allowed to proliferate for one week followed by osteogenic induction. Staining for live/dead cells as well as scanning electron microscopy (SEM) was carried out to determine viability and cellular binding. Further outcome measures included alkaline phosphatase (ALP) assays with normalisation for DNA content to quantify osteogenic potential. Negative and positive control experiments were carried out in parallel to validate the results.
Live/dead and SEM imaging showed higher viability and attachment with β-tricalcium phosphate (β-TCP) than with other BGS (P < 0.05). The average ALP activity in nmol/mL (normalised value for DNA content in nmol/μg DNA) per sample was 657.58 (132.03) for β-TCP, 36.22 (unable to normalise) for calcium sulphate, 19.93 (11.39) for the Hydroxyapatite/Tricalcium Phosphate composite, 14.79 (18.53) for polygraft, 13.98 (8.15) for the highly porous β-Tricalcium Phosphate, 5.56 (10.0) for polymers, and 3.82 (3.8) for Hydroxyapatite.
Under the above experimental conditions, β-TCP was able to maintain better the viability of osteoprogenitor cells and allow proliferation and differentiation (P < 0.05).
在不使用任何额外生物生长因子的情况下，比较七种市售骨移植替代物（BGS）的这些特性。
将猪成骨祖细胞接种到七种市售BGS上，使其增殖一周，然后进行成骨诱导。进行活/死细胞染色以及扫描电子显微镜（SEM）检查以确定细胞活力和细胞黏附情况。进一步的结果指标包括碱性磷酸酶（ALP）测定，并对DNA含量进行标准化以量化成骨潜力。同时进行阴性和阳性对照实验以验证结果。
活/死细胞成像和SEM成像显示，与其他BGS相比，β-磷酸三钙（β-TCP）具有更高的细胞活力和细胞附着率（P < 0.05）。每个样品中以nmol/mL为单位的平均ALP活性（针对nmol/μg DNA中的DNA含量进行标准化的值），β-TCP为657.58（132.03），硫酸钙为36.22（无法标准化），羟基磷灰石/磷酸三钙复合物为19.93（11.39），多聚体为14.79（18.53），高度多孔β-磷酸三钙为13.98（8.15），聚合物为5.56（10.0），羟基磷灰石为3.82（3.8）。
在上述实验条件下，β-TCP能够更好地维持成骨祖细胞的活力，并促进其增殖和分化（P < 0.05）。
