纯化的脂皮质素在小鼠体内具有与糖皮质激素相同的抗炎作用。
A purified lipocortin shares the anti-inflammatory effect of glucocorticosteroids in vivo in mice.
作者信息
Errasfa M, Russo-Marie F
机构信息
Unité associée INSERM no. 285/Institut Pasteur, Paris, France.
出版信息
Br J Pharmacol. 1989 Aug;97(4):1051-8. doi: 10.1111/j.1476-5381.1989.tb12561.x.
Abstract
- The injection of a suspension of a polyacrylamide gel (bio gel) into the dorsal subcutaneous area of mice induced an inflammatory reaction and the migration of neutrophils towards the inflamed site. 2. The intravenous administration of anti-inflammatory drugs (dexamethasone, indomethacin and lysine-acetylsalicylate) to polyacrylamide gel-treated mice inhibited the accumulation of neutrophils in the inflamed site. 3. A similar administration of a 36K mouse lipocortin, induced a strong dose-dependent inhibition of neutrophil accumulation in the inflamed site. 4. Dexamethasone and lipocortin inhibited the production of eicosanoids, prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) in the inflamed site of polyacrylamide gel-treated mice. 5. Lipocortin impaired both phospholipase A2 (PLA2) activity and chemotaxis of isolated inflammatory neutrophils. 6. The present studies show an in vivo anti-inflammatory effect of lipocortin similar to that of glucocorticosteroids. In agreement with recent data on the extracellular effects of various lipocortins, these results might implicate lipocortin(s) in the anti-inflammatory effects of glucocorticosteroids.
摘要
- 向小鼠背部皮下区域注射聚丙烯酰胺凝胶（生物凝胶）悬液会引发炎症反应，并使中性粒细胞向炎症部位迁移。2. 对经聚丙烯酰胺凝胶处理的小鼠静脉注射抗炎药物（地塞米松、吲哚美辛和赖氨酸-乙酰水杨酸）可抑制中性粒细胞在炎症部位的聚集。3. 类似地给予36K小鼠脂皮质素，可在炎症部位对中性粒细胞的聚集产生强烈的剂量依赖性抑制作用。4. 地塞米松和脂皮质素可抑制经聚丙烯酰胺凝胶处理的小鼠炎症部位类花生酸、前列腺素E2（PGE2）和白三烯B4（LTB4）的产生。5. 脂皮质素会损害分离出的炎症中性粒细胞的磷脂酶A2（PLA2）活性和趋化性。6. 本研究表明脂皮质素具有与糖皮质激素类似的体内抗炎作用。与近期关于各种脂皮质素细胞外效应的数据一致，这些结果可能表明脂皮质素参与了糖皮质激素的抗炎作用。
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