Fensterer Tathyana F, Miller Cynthia J, Perez-Abadia Gustavo, Maldonado Claudio
Department of Surgery, University of Louisville, Louisville, Kentucky, USA.
Department of Physiology and Biophysics, University of Louisville, Louisville, Kentucky, USA.
Comp Med. 2014 Aug;64(4):293-9.
Cervical heterotopic heart transplantation in rodents is a useful tool for studying transplantation immunology. However, end-to-end anastomosis of small-diameter vessels by using standard microsurgical technique is technically difficult and can require prolonged graft ischemia. A novel cuff system was designed from polyethylene tubing to allow anastomosis of vessels with internal luminal diameters of 0.3 to 0.9 mm. Key features include a spring-like adjustable lumen to facilitate vessel eversion, a barb to hold vessel ends in place after eversion, and a handling system that allows easy manipulation and stabilization of cuffs by a single operator. After a training period, a single operator performed a series of 8 transplants in which the mean warm ischemic time of grafts was 8.5 ± 2.9 min. Here we provide a detailed description of how to construct and perform end-to-end vessel anastomosis by using our novel cuff system. The discussion of the technique is supplemented with tips learned during the process of developing a reliable experimental model.
啮齿动物颈部异位心脏移植是研究移植免疫学的一种有用工具。然而，使用标准显微外科技术进行小直径血管的端端吻合在技术上具有挑战性，并且可能需要延长移植物缺血时间。一种新型袖套系统由聚乙烯管设计而成，可用于内径为0.3至0.9毫米的血管吻合。关键特征包括一个类似弹簧的可调节管腔，以利于血管外翻；一个倒刺，用于在血管外翻后固定血管末端；以及一个操作系统，允许单个操作者轻松操作和稳定袖套。经过一段时间的训练后，一名操作者进行了一系列8次移植手术，移植物的平均热缺血时间为8.5±2.9分钟。在此，我们详细描述如何使用我们的新型袖套系统构建和进行血管端端吻合。在开发可靠实验模型的过程中所学到的技巧也补充到了对该技术的讨论中。
