Takehara Hiroaki, Nagaoka Akira, Noguchi Jun, Akagi Takanori, Kasai Haruo, Ichiki Takanori
Department of Bioengineering, School of Engineering, The University of Tokyo, 2-11-16, Yayoi, Bunkyo-ku, Tokyo. 113-8656, Japan.
Laboratory of Structural Physiology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo. 113-0033, Japan.
Sci Rep. 2014 Oct 22;4:6721. doi: 10.1038/srep06721.
The high-resolution imaging of neural cells in vivo has brought about great progress in neuroscience research. Here, we report a novel experimental platform, where the intact brain of a living mouse can be studied with the aid of a surgically implanted micro-optical fluidic device; acting as an interface between neurons and the outer world. The newly developed device provides the functions required for the long-term and high-resolution observation of the fine structures of neurons by two-photon laser scanning microscopy and the microfluidic delivery of chemicals or drugs directly into the brain. A proof-of-concept experiment of single-synapse stimulation by two-photon uncaging of caged glutamate and observation of dendritic spine shrinkage over subsequent days demonstrated a promising use for the present technology.
体内神经细胞的高分辨率成像在神经科学研究中取得了巨大进展。在此，我们报告了一个新颖的实验平台，借助手术植入的微光学流体装置，可以对活体小鼠的完整大脑进行研究；该装置充当神经元与外部世界之间的接口。新开发的装置提供了通过双光子激光扫描显微镜对神经元精细结构进行长期高分辨率观察以及将化学物质或药物直接微流体递送至大脑所需的功能。通过双光子解开笼锁谷氨酸对单突触进行刺激并在随后几天观察树突棘收缩的概念验证实验证明了该技术具有广阔的应用前景。
