Parfitt David-Emlyn, Shen Michael M
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA Department of Urology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA Department of Systems Biology, Herbert Irving Comprehensive Cancer Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA Department of Urology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA Department of Systems Biology, Herbert Irving Comprehensive Cancer Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Philos Trans R Soc Lond B Biol Sci. 2014 Dec 5;369(1657). doi: 10.1098/rstb.2013.0542.
To date, many regulatory genes and signalling events coordinating mammalian development from blastocyst to gastrulation stages have been identified by mutational analyses and reverse-genetic approaches, typically on a gene-by-gene basis. More recent studies have applied bioinformatic approaches to generate regulatory network models of gene interactions on a genome-wide scale. Such models have provided insights into the gene networks regulating pluripotency in embryonic and epiblast stem cells, as well as cell-lineage determination in vivo. Here, we review how regulatory networks constructed for different stem cell types relate to corresponding networks in vivo and provide insights into understanding the molecular regulation of the blastocyst-gastrula transition.
迄今为止，通过突变分析和反向遗传学方法，通常是逐个基因地，已经鉴定出了许多在从囊胚到原肠胚形成阶段协调哺乳动物发育的调控基因和信号事件。最近的研究应用生物信息学方法在全基因组范围内生成基因相互作用的调控网络模型。这些模型为调控胚胎和上胚层干细胞多能性的基因网络以及体内细胞谱系的确定提供了见解。在这里，我们回顾了针对不同干细胞类型构建的调控网络如何与体内相应网络相关，并为理解囊胚-原肠胚转变的分子调控提供见解。
