发根农杆菌rolA、rolB和rolC基因的启动子在转基因植物中受到不同的调控。
Promoters of the rolA, B, and C genes of Agrobacterium rhizogenesare differentially regulated in transgenic plants.
作者信息
Schmülling T, Schell J, Spena A
机构信息
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
出版信息
Plant Cell. 1989 Jul;1(7):665-70. doi: 10.1105/tpc.1.7.665.
Abstract
Chimeric genes containing the beta-glucuronidase reporter gene under the control of the rolA, B, and C promoters of Agrobacterium rhizogenes are expressed in a regulated manner in transgenic plants. The intergenic region separating the rolB and C genes represents a bidirectional promoter. This bidirectional promoter regulates transcription for both genes in a similar fashion in aerial organs of the plants, but in a distinct way in roots. Moreover, both rolB and C promoter activities differ from those characteristic of the rolA promoter. Thus, promoters of bacterial origin show differential expression in transgenic plants, and regulation of roI gene expression plays a role in the biological effects caused by the rolA, B, and C genes.
摘要
含有在发根农杆菌rolA、B和C启动子控制下的β-葡萄糖醛酸酶报告基因的嵌合基因在转基因植物中以一种受调控的方式表达。分隔rolB和C基因的基因间区域代表一个双向启动子。这个双向启动子在植物地上器官中以类似方式调控两个基因的转录，但在根中则以不同方式调控。此外，rolB和C启动子活性均不同于rolA启动子的特征活性。因此，细菌来源的启动子在转基因植物中表现出差异表达，并且rol基因表达的调控在由rolA、B和C基因引起的生物学效应中发挥作用。
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