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本文引用的文献

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Inheritance of functional foreign genes in plants.植物中外源基因的功能遗传。
Science. 1984 Feb 3;223(4635):496-8. doi: 10.1126/science.223.4635.496.
2
Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype.能够诱导毛状根表型的发根农杆菌T-DNA基因。
Mol Gen Genet. 1987 Oct;209(3):475-80. doi: 10.1007/BF00331152.
3
Independent and synergistic activity of rol A, B and C loci in stimulating abnormal growth in plants.rolA、B 和 C 基因座在刺激植物异常生长中的独立和协同活性。
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Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes.发根农杆菌致根质粒转移DNA区域的分子与遗传分析
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5
rolA locus of the Ri plasmid directs developmental abnormalities in transgenic tobacco plants.发根农杆菌Ri质粒的rolA基因座导致转基因烟草植株发育异常。
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6
Versatile cloning vectors for transient gene expression and direct gene transfer in plant cells.用于植物细胞中瞬时基因表达和直接基因转移的多功能克隆载体。
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7
Structure and expression of Ri T-DNA from Agrobacterium rhizogenes in Nicotiana tabacum. Organ and phenotypic specificity.发根农杆菌Ri T-DNA在烟草中的结构与表达。器官及表型特异性。
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
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发根农杆菌rolA、rolB和rolC基因的启动子在转基因植物中受到不同的调控。

Promoters of the rolA, B, and C genes of Agrobacterium rhizogenesare differentially regulated in transgenic plants.

作者信息

Schmülling T, Schell J, Spena A

机构信息

Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.

出版信息

Plant Cell. 1989 Jul;1(7):665-70. doi: 10.1105/tpc.1.7.665.

DOI:10.1105/tpc.1.7.665
PMID:2535517
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC159802/
Abstract

Chimeric genes containing the beta-glucuronidase reporter gene under the control of the rolA, B, and C promoters of Agrobacterium rhizogenes are expressed in a regulated manner in transgenic plants. The intergenic region separating the rolB and C genes represents a bidirectional promoter. This bidirectional promoter regulates transcription for both genes in a similar fashion in aerial organs of the plants, but in a distinct way in roots. Moreover, both rolB and C promoter activities differ from those characteristic of the rolA promoter. Thus, promoters of bacterial origin show differential expression in transgenic plants, and regulation of roI gene expression plays a role in the biological effects caused by the rolA, B, and C genes.

摘要

含有在发根农杆菌rolA、B和C启动子控制下的β-葡萄糖醛酸酶报告基因的嵌合基因在转基因植物中以一种受调控的方式表达。分隔rolB和C基因的基因间区域代表一个双向启动子。这个双向启动子在植物地上器官中以类似方式调控两个基因的转录,但在根中则以不同方式调控。此外,rolB和C启动子活性均不同于rolA启动子的特征活性。因此,细菌来源的启动子在转基因植物中表现出差异表达,并且rol基因表达的调控在由rolA、B和C基因引起的生物学效应中发挥作用。