Tanaka A, Noda T, Yajima H, Hatanaka M, Ito Y
Institute for Virus Research, Kyoto University, Japan.
J Virol. 1989 Mar;63(3):1465-9. doi: 10.1128/JVI.63.3.1465-1469.1989.
Previously, we observed sequential two-step alteration, growth stimulation, and progression to a more malignant state in NIH 3T3 cells transfected by human papillomavirus type 16 (HPV-16) DNA. In this study, we prepared a cDNA library from RNA extracted from cells transfected with the HPV-16 DNA and isolated cDNA clones which had growth-stimulating activity. Analysis of these cDNA clones indicated that the E7 open reading frame alone is responsible for inducing both steps of this cell transformation.
此前，我们观察到，用16型人乳头瘤病毒（HPV - 16）DNA转染的NIH 3T3细胞中，会出现连续两步的改变、生长刺激，并进展为更具恶性的状态。在本研究中，我们从用HPV - 16 DNA转染的细胞中提取的RNA制备了一个cDNA文库，并分离出具有生长刺激活性的cDNA克隆。对这些cDNA克隆的分析表明，仅E7开放阅读框就负责诱导这种细胞转化的两个步骤。
