采用多元统计分析数据集对白参产地的鉴别。
Discrimination of white ginseng origins using multivariate statistical analysis of data sets.
机构信息
Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Korea.
Experiment Research Institute of National Agricultural Products Quality Management Service, Gimcheon, Korea.
出版信息
J Ginseng Res. 2014 Jul;38(3):187-93. doi: 10.1016/j.jgr.2014.03.002. Epub 2014 Apr 3.
BACKGROUND
White ginseng (Panax ginseng Meyer) is commonly distributed as a health food in food markets. However, there is no practical method for distinguishing Korean white ginseng (KWG) from Chinese white ginseng (CWG), except for relying on the traceability system in the market.
METHODS
Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry combined with orthogonal partial least squares discrimination analysis (OPLS-DA) was employed to discriminate between KWG and CWG.
RESULTS
The origins of white ginsengs in two test sets (1.0 μL and 0.2 μL injections) could be successfully discriminated by the OPLS-DA analysis. From OPLS-DA S-plots, KWG exhibited tentative markers derived from ginsenoside Rf and notoginsenoside R3 isomer, whereas CWG exhibited tentative markers derived from ginsenoside Ro and chikusetsusaponin Iva.
CONCLUSION
Results suggest that ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry coupled with OPLS-DA is an efficient tool for identifying the difference between the geographical origins of white ginsengs.
背景
白参（Panax ginseng Meyer）作为一种保健食品在食品市场上广泛分布。然而，除了依靠市场上的可追溯系统之外，目前还没有实际的方法来区分高丽白参（KWG）和中国白参（CWG）。
方法
采用超高效液相色谱四极杆飞行时间质谱联用正交偏最小二乘判别分析（OPLS-DA）区分 KWG 和 CWG。
结果
OPLS-DA 分析能够成功区分两个测试集（1.0 μL 和 0.2 μL 进样）中白参的产地。从 OPLS-DA S-plot 可以看出，KWG 表现出源于人参皂苷 Rf 和三七皂苷 R3 异构体的特征标记物，而 CWG 则表现出源于人参皂苷 Ro 和远志皂苷 Iva 的特征标记物。
结论
结果表明，超高效液相色谱四极杆飞行时间质谱联用 OPLS-DA 是一种有效工具，可用于鉴定白参产地之间的差异。
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