Morabbi Najafabad Amin, Jamei Rashid
Department of Biology, Faculty of Science, Urmia University, Urmia, I. R. Iran.
Avicenna J Phytomed. 2014 Sep;4(5):343-53.
Consumption of fruits, such as plums and prunes, is useful in treating blood circulation disorder, measles, digestive disorder, and prevention of cancer, diabetes, and obesity. The paper presents a description of antioxidant and antiradical capacity of plum (Prunus domestica L.) in both fresh and dried samples.
Samples were mixed with methanol and ethanol (as solvents) and were extracted on magnetic shaker, separately. The experiments were carried out to measure the Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total Antioxidant Capacity (TAC), Reducing Power Assay (RPA), Chain Breaking Activity (CBA), and quantity of Malondialdehyde (MDA), 2,2-Diphenyl-1-Picrylhydrazyl (DPPH),Nitric Oxide (NO),Hydrogen peroxide (H2O2) and superoxide(O2 (-)) radicals inhibition.
The results showed that the highest values for the TPC, TFC,TAC, RPA, CBA, DPPH, and NO were related to ethanolic extractsof dried sample which showed statistically significant differences (p<0.01 and p<0.0001), while the maximum values for the H2O2 and O2 (-)were related to ethanolic extracts of fresh sample. The correlations data were analyzed among all parameters and the TPC and TFC had a significant correlation (r(2)=0.977). Moreover, it was found that methanol was more successful in extraction procedure than ethanol (p<0.01).
Findings suggest that the fresh samples are more successful in collecting oxygen free radicals such as superoxide (O2 (-)) and peroxy radicals (ROO(.)) than dried.
食用李子和西梅等水果有助于治疗血液循环紊乱、麻疹、消化紊乱，以及预防癌症、糖尿病和肥胖症。本文介绍了新鲜和干燥李子（欧洲李）样本的抗氧化和抗自由基能力。
样本分别与甲醇和乙醇（作为溶剂）混合，并在磁力搅拌器上进行提取。进行实验以测量总酚含量（TPC）、总黄酮含量（TFC）、总抗氧化能力（TAC）、还原力测定（RPA）、链断裂活性（CBA）以及丙二醛（MDA）、2,2-二苯基-1-苦基肼（DPPH）、一氧化氮（NO）、过氧化氢（H2O2）和超氧阴离子（O2(-)）自由基的抑制量。
结果表明，干燥样本的乙醇提取物中TPC、TFC、TAC、RPA、CBA、DPPH和NO的值最高，显示出统计学上的显著差异（p<0.01和p<0.0001），而H2O2和O2(-)的最大值与新鲜样本的乙醇提取物有关。对所有参数之间的相关性数据进行分析，发现TPC和TFC具有显著相关性（r(2)=0.977）。此外，发现甲醇在提取过程中比乙醇更成功（p<0.01）。
研究结果表明，新鲜样本在收集超氧阴离子（O2(-)）和过氧自由基（ROO(.)）等氧自由基方面比干燥样本更成功。
