Institute of Chemistry and Nano-Science Center, University of Copenhagen, Universitetparken 5, DK 2100, Copenhagen, Denmark.
Soft Matter. 2013 Mar 27;9(16):4219-26. doi: 10.1039/c3sm27769k.
Soluble in the extracellular matrix experience a crowded environment. However, most of the biophysical studies performed to date have focused on concentrations within the dilute regime (well below the mM range). Here, we systematically studied the interaction of model cell membrane systems (giant unilamellar vesicles and supported bilayers) with soluble globular , bovine serum albumin, and lysozyme at physiologically relevant concentrations. To mimic the extracellular environment more closely, we also used fetal bovine serum as a good representative of a biomimetic mixture. We found that regardless of the used (and thus of their biological function), the interactions between a model cell membrane and these are determined by their physico-chemical characteristics, mainly their dipolar character (or charged patches). In this paper we discuss the specificity and reversibility of these interactions and their potential implications on the living cells. In particular, we report initial evidence for an additional role of in cell membranes: that of reducing the effects of non-specific of soluble on the cell membrane.
可溶蛋白在细胞外基质中经历拥挤的环境。然而，迄今为止进行的大多数生物物理研究都集中在稀释范围内的浓度上（远低于 mM 范围）。在这里，我们系统地研究了模型细胞膜系统（巨大的单层囊泡和支撑的双层膜）与可溶性球状蛋白、牛血清白蛋白和溶菌酶在生理相关浓度下的相互作用。为了更紧密地模拟细胞外环境，我们还使用胎牛血清作为仿生混合物的良好代表。我们发现，无论使用何种（因此具有其生物功能），模型细胞膜与这些蛋白之间的相互作用取决于它们的物理化学特性，主要是它们的偶极特性（或带电斑块）。在本文中，我们讨论了这些相互作用的特异性和可逆性及其对活细胞的潜在影响。特别是，我们报告了在细胞膜中存在额外作用的初步证据：即在细胞膜上减少可溶性非特异性的影响。
