Yariv Inbar, Rahamim Gilad, Shliselberg Elad, Duadi Hamootal, Lipovsky Anat, Lubart Rachel, Fixler Dror
Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Ramat Gan 5290002, Israel.
Physics and Chemistry Department, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Biomed Opt Express. 2014 Oct 10;5(11):3871-81. doi: 10.1364/BOE.5.003871. eCollection 2014 Nov 1.
Determining the physical penetration depth of nanoparticles (NPs) into tissues is a challenge that many researchers have been facing in recent years. This paper presents a new noninvasive method for detecting NPs in tissue using an optical iterative technique based on the Gerchberg-Saxton (G-S) algorithm. At the end of this algorithm the reduced scattering coefficient (µs'), of a given substance, can be estimated from the standard deviation (STD) of the retrieved phase of the remitted light. Presented in this paper are the results of a tissue simulation which indicate a linear ratio between the STD and the scattering components. A linear ratio was also observed in the tissue-like phantoms and in ex vivo experiments with and without NPs (Gold nanorods and nano Methylene Blue). The proposed technique is the first step towards determining the physical penetration depth of NPs.
确定纳米颗粒（NPs）在组织中的物理穿透深度是近年来许多研究人员一直面临的挑战。本文提出了一种基于格尔奇伯格 - 萨克斯顿（G - S）算法的光学迭代技术，用于在组织中检测纳米颗粒的新型非侵入性方法。在该算法结束时，可以从发射光的检索相位的标准偏差（STD）估计给定物质的约化散射系数（µs'）。本文展示了组织模拟的结果，这些结果表明STD与散射成分之间存在线性比例关系。在类组织体模以及有和没有纳米颗粒（金纳米棒和纳米亚甲蓝）的离体实验中也观察到了线性比例关系。所提出的技术是确定纳米颗粒物理穿透深度的第一步。
