Lubart Rachel, Yariv Inbar, Fixler Dror, Lipovsky Anat
Prof. Lubart and Dr. Lipovsky are with the Chemistry Department at Bar-Ilan University in Ramat-Gan, Israel. Prof. Lubart is also with the Physics Department at Bar-Ilan University in Ramat-Gan, Israel.
Ms. Yariv and Prof.. Fixler are with the Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University in Ramat-Gan, Israel.
J Clin Aesthet Dermatol. 2019 Oct;12(10):39-44. Epub 2019 Oct 1.
Topical hyaluronic acid (HA) has shown effectiveness in maintaining skin hydration. Topical creams containing HA are widely available, but their efficacy is limited by their lack of penetration into the skin due to the large molecule size of HA, the result of being formulated into a cream base. In this three-part study (, and ), molecule sizes, penetration levels, and antiaging qualities of a topical HA facial cream that was formulated using a new technology that micronizes HA molecules (m-HA) were assessed. Particle sizes of m-HA were evaluated using electron microscopy, which showed varying sizes, the smallest of which was 100nm in diameter. The antioxidation capabilities of m-HA were measured using electron spin resonance and were found to be higher than original HA. Skin penetration of the m-HA formulation was evaluated via immunohistochemical staining of porcine skin samples, which demonstrated penetration of the formulation into the stratum corneum and the deep epidermal layers toward the dermis. Antiaging qualities of the m-HA formulation were assessed in an open-label clinical study that included 36 healthy adult women. Skin parameters were measured objectively (e.g., Corneometer, Cutometer) and subjectively via patient questionnaire, results of which indicated significant improvements in facial skin hydration, elasticity, and wrinkle depth. The topical HA facial cream with m-HA technology demonstrated penetration into the epidermal skin layer, and, to our knowledge, our formulation is the first HA facial cream to achieve this. Clinical application of the facial cream demonstrated objective and subjective improvements in facial skin quality of healthy adult female subjects. Our results support the use of this new HA facial cream with m-HA technology as an effective antiaging topical therapy. Larger randomized, controlled studies are needed to confirm our findings.
局部外用透明质酸（HA）已显示出在维持皮肤水分方面的有效性。含有HA的局部乳膏广泛可得，但其功效受到限制，因为HA分子量大，制成乳膏基质后难以渗透到皮肤中。在这个分为三个部分的研究（……以及……）中，对一种采用新技术将HA分子微粉化（m-HA）制成的局部HA面霜的分子大小、渗透水平和抗衰老特性进行了评估。使用电子显微镜评估了m-HA的粒径，结果显示粒径大小不一，其中最小的直径为100nm。使用电子自旋共振测量了m-HA的抗氧化能力，发现其高于原始HA。通过对猪皮肤样本进行免疫组织化学染色评估了m-HA制剂的皮肤渗透性，结果表明该制剂可渗透到角质层并深入表皮层直至真皮。在一项包括36名健康成年女性的开放标签临床研究中评估了m-HA制剂的抗衰老特性。通过客观测量（如皮肤水分测试仪、皮肤弹性测试仪）和患者问卷调查主观评估皮肤参数，结果表明面部皮肤的水分含量、弹性和皱纹深度有显著改善。采用m-HA技术的局部HA面霜证明可渗透到表皮皮肤层，据我们所知，我们的制剂是首款能做到这一点的HA面霜。该面霜的临床应用表明，健康成年女性受试者的面部皮肤质量在客观和主观上都有改善。我们的结果支持将这种采用m-HA技术的新型HA面霜作为一种有效的抗衰老局部治疗方法。需要进行更大规模的随机对照研究来证实我们的发现。
