Center for Green Chemistry and Green Engineering at Yale, 225 Prospect Street, New Haven, CT 06520, United States; Department of Chemical and Environmental Engineering, Yale University, 9 Hillhouse Avenue, New Haven, CT 06511, United States.
Center for Green Chemistry and Green Engineering at Yale, 225 Prospect Street, New Haven, CT 06520, United States.
Bioresour Technol. 2014 Dec;173:415-421. doi: 10.1016/j.biortech.2014.09.048. Epub 2014 Sep 19.
Carbohydrate composition of the marine microalgae, Tetraselmis suecica was characterized following acidic and enzymatic hydrolysis. Monitoring intracellular starch as a function of cultivation time at varying nitrate concentrations showed a maximum cellular starch content of 45% of dry biomass when grown under nitrate depleted conditions. Characterization of the cell wall methanolysates using GC/MS showed that the monosaccharide composition did not change in response to the nitrate concentration and consisted of 54% 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), 17% 3-deoxy-lyxo-2-heptulosaric acid (Dha), 21% galacturonic acid and 6% galactose. Presence of up to 5% Kdo in the dry weight of T. suecica established in this study demonstrates the potential of the cell wall of this species as a feedstock for Kdo, a sugar that is difficult to obtain by chemical synthesis and that has applications in medicinal chemistry.
采用酸解和酶解的方法对海洋微藻三角褐指藻的碳水化合物组成进行了分析。监测不同硝氮浓度条件下培养时间对细胞内淀粉的影响，结果表明，在硝氮耗尽的条件下培养时，细胞内淀粉的最大含量为干重的 45%。采用 GC/MS 对细胞壁甲醇提取物进行了分析，结果表明，单糖组成并没有随硝氮浓度的变化而改变，主要包括 54%的 3-脱氧-D-甘露-2-辛酮糖酸（Kdo）、17%的 3-脱氧-L-赤藓-2-庚酮糖酸（Dha）、21%的半乳糖醛酸和 6%的半乳糖。本研究中，三角褐指藻干重中 Kdo 的含量高达 5%，这表明该物种的细胞壁有可能成为 Kdo 的一种原料，Kdo 是一种难以通过化学合成获得的糖，在药物化学中有多种用途。
