Martin A, Bovill W D, Percy C D, Herde D, Fletcher S, Kelly A, Neate S M, Sutherland M W
Centre for Crop Health, University of Southern Queensland, Toowoomba, QLD, 4350, Australia,
Theor Appl Genet. 2015 Mar;128(3):377-85. doi: 10.1007/s00122-014-2437-1. Epub 2014 Dec 4.
QTL identified for seedling and adult plant crown rot resistance in four partially resistant hexaploid wheat sources. PCR-based markers identified for use in marker-assisted selection. Crown rot, caused by Fusarium pseudograminearum, is an important disease of wheat in many wheat-growing regions globally. Complete resistance to infection by F. pseudograminearum has not been observed in a wheat host, but germplasm with partial resistance to this pathogen has been identified. The partially resistant wheat hexaploid germplasm sources 2-49, Sunco, IRN497 and CPI133817 were investigated in both seedling and adult plant field trials to identify markers associated with the resistance which could be used in marker-assisted selection programs. Thirteen different quantitative trait loci (QTL) conditioning crown rot resistance were identified in the four different sources. Some QTL were only observed in seedling trials whereas others appeared to be adult plant specific. For example while the QTL on chromosomes 1AS, 1BS, and 4BS contributed by 2-49 and on 2BS contributed by Sunco were detected in both seedling and field trials, the QTL on 1DL present in 2-49 and the QTL on 3BL in IRN497 were only detected in seedling trials. Genetic correlations between field trials of the same population were strong, as were correlations between seedling trials of the same population. Low to moderate correlations were observed between seedling and field trials. Flanking markers, most of which are less than 10 cM apart, have now been identified for each of the regions associated with crown rot resistance.
在四个部分抗病的六倍体小麦种质资源中鉴定出与幼苗和成株冠腐病抗性相关的QTL。鉴定出基于PCR的标记用于标记辅助选择。由拟禾本科镰刀菌引起的冠腐病是全球许多小麦种植区小麦的一种重要病害。在小麦寄主中尚未观察到对拟禾本科镰刀菌感染的完全抗性，但已鉴定出对该病原菌具有部分抗性的种质。对部分抗病的六倍体小麦种质资源2-49、Sunco、IRN497和CPI133817进行了幼苗和成株田间试验，以鉴定与抗性相关的标记，这些标记可用于标记辅助选择计划。在四个不同的种质资源中鉴定出13个不同的调控冠腐病抗性的数量性状位点（QTL）。一些QTL仅在幼苗试验中观察到，而其他QTL似乎是成株特异性的。例如，由2-49贡献的位于1AS、1BS和4BS染色体上的QTL以及由Sunco贡献的位于2BS染色体上的QTL在幼苗试验和田间试验中均被检测到，而2-49中存在的位于1DL染色体上的QTL和IRN497中位于3BL染色体上的QTL仅在幼苗试验中被检测到。同一群体的田间试验之间的遗传相关性很强，同一群体的幼苗试验之间的相关性也很强。在幼苗试验和田间试验之间观察到低至中等的相关性。现在已经为与冠腐病抗性相关的每个区域鉴定出侧翼标记，其中大多数侧翼标记之间的距离小于10厘摩。
