Norqvist A, Hagström A, Wolf-Watz H
Department of Microbiology, University of Umeå, Sweden.
Appl Environ Microbiol. 1989 Jun;55(6):1400-5. doi: 10.1128/aem.55.6.1400-1405.1989.
The fish pathogen Vibrio anguillarum causes a lethal infection in rainbow trout (Salmo gairdneri). Three different avirulent mutants, constructed by transposon insertion mutagenesis (VAN20 and VAN70) or as antibiotic-resistant mutants (VAN1000), were isolated by screening 200 individual isolated mutants for avirulence. When used as live vaccines, all three avirulent mutants were able to induce protective immunity against the homologous as well as a heterologous strain of V. anguillarum. When VAN1000 was used, protective immunity could be recorded 1 week after bath vaccination with 10(7) bacteria per ml of water for 30 min. A single-dose immunization was effective for at least 12 weeks. Western immunoblotting showed that strains of V. anguillarum have antigenic determinants in common with Aeromonas strains. Therefore, we tested and confirmed that VAN1000 also was able to induce protective immunity against challenge with Aeromonas salmonicida.
鱼类病原菌鳗弧菌可在虹鳟（Salmo gairdneri）中引起致死性感染。通过转座子插入诱变构建了三种不同的无毒突变体（VAN20和VAN70），或作为抗生素抗性突变体（VAN1000），通过筛选200个单独分离的突变体的无毒力特性来分离得到这些突变体。当用作活疫苗时，所有三种无毒突变体都能够诱导针对同源以及异源鳗弧菌菌株的保护性免疫。当使用VAN1000时，在每毫升水含10(7) 个细菌的条件下进行30分钟浸浴接种后1周即可记录到保护性免疫。单剂量免疫至少12周有效。蛋白质免疫印迹表明，鳗弧菌菌株与气单胞菌菌株具有共同的抗原决定簇。因此，我们测试并证实VAN1000也能够诱导针对杀鲑气单胞菌攻击的保护性免疫。
