Woisetschlaeger M, Strominger J L, Speck S H
Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, MA 02115.
Proc Natl Acad Sci U S A. 1989 Sep;86(17):6498-502. doi: 10.1073/pnas.86.17.6498.
Of the eight viral antigens known to be expressed during Epstein-Barr virus latency, six are transcribed from a major rightward transcriptional unit, which gives rise to mRNAs containing common 5' exons. Analysis of cDNA clones has identified the use of two different promoters (Wp and Cp), located near the left-hand end of the viral genome, in generating these viral messages. Characterization of the activities of these two viral promoters in a number of Burkitt lymphoma and lymphoblastoid cell lines has revealed exclusive usage of only one of these promoters in all cell lines examined. Transfection of reporter constructs containing Wp and/or Cp linked to the bacterial chloramphenicol acetyltransferase gene into several different Epstein-Barr virus-infected cell lines generally supports a model in which the mutually exclusive use of Cp or Wp is determined by cellular factors and not by viral strain variation.
在已知的爱泼斯坦-巴尔病毒潜伏期间表达的八种病毒抗原中，有六种是从一个主要的向右转录单元转录而来的，该转录单元产生含有共同5'外显子的mRNA。对cDNA克隆的分析已确定在产生这些病毒信息时使用了位于病毒基因组左端附近的两个不同启动子（Wp和Cp）。对这两个病毒启动子在许多伯基特淋巴瘤和淋巴母细胞系中的活性进行表征后发现，在所检测的所有细胞系中仅排他性地使用其中一个启动子。将含有与细菌氯霉素乙酰转移酶基因相连的Wp和/或Cp的报告构建体转染到几种不同的爱泼斯坦-巴尔病毒感染细胞系中，总体上支持一种模型，即Cp或Wp的互斥使用是由细胞因子而非病毒株变异决定的。
