尿pH值与空斑小室技术检测巨细胞病毒的相关性
Correlation of urine pH with the detection of cytomegalovirus by the shell vial technique.
作者信息
Gray L D, Ilstrup D M, Smith T F
机构信息
Section of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota 55905.
出版信息
Diagn Microbiol Infect Dis. 1989 May-Jun;12(3):275-7. doi: 10.1016/0732-8893(89)90028-x.
Abstract
The influence of the pH of urine on the detection of cytomegalovirus (CMV) by the shell vial assay was evaluated. The pH values of 295 urine specimens ranged from 4.7 to 8.5 (mean 6.3) and were not significantly different in culture-positive versus culture-negative samples. None of the urine specimens appeared to be toxic for the cells used in the shell vial assay. We recommend inoculation of urine specimens into shell vials without adjustment of pH.
摘要
评估了尿液pH值对空斑试验检测巨细胞病毒（CMV）的影响。295份尿液标本的pH值范围为4.7至8.5（平均6.3），培养阳性和培养阴性样本之间无显著差异。所有尿液标本对空斑试验所用细胞均无毒性。我们建议在不调节pH值的情况下将尿液标本接种到空斑试验瓶中。
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本文引用的文献
1
Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.通过低速离心和使用针对早期抗原的单克隆抗体，快速检测接种尿液标本的MRC-5细胞中的巨细胞病毒。
J Clin Microbiol. 1984 Jun;19(6):917-9. doi: 10.1128/jcm.19.6.917-919.1984.
2
Immunological detection of cytomegalovirus early antigen on monolayers inoculated with urine specimens by centrifugation and cultured for 6 days as alternative to conventional virus isolation.通过离心接种尿液标本的单层细胞并培养6天，以免疫方法检测巨细胞病毒早期抗原，作为传统病毒分离方法的替代方法。
J Clin Microbiol. 1988 Jul;26(7):1313-5. doi: 10.1128/jcm.26.7.1313-1315.1988.
3
Rapid detection of cytomegalovirus by fluorescent monoclonal antibody staining and in situ DNA hybridization in a dram vial cell culture system.在微量培养瓶细胞培养系统中通过荧光单克隆抗体染色和原位DNA杂交快速检测巨细胞病毒
J Clin Microbiol. 1988 Jun;26(6):1111-4. doi: 10.1128/jcm.26.6.1111-1114.1988.
4
Detection of cytomegalovirus in clinical specimens by virus isolation and by a monoclonal antibody against the early nuclear antigen.通过病毒分离和针对早期核抗原的单克隆抗体检测临床标本中的巨细胞病毒。
J Med Virol. 1988 Mar;24(3):275-82. doi: 10.1002/jmv.1890240305.
5
Comparative study of inactivation of herpes simplex virus types 1 and 2 by commonly used antiseptic agents.常用抗菌剂对1型和2型单纯疱疹病毒灭活作用的比较研究
J Clin Microbiol. 1988 Feb;26(2):213-5. doi: 10.1128/jcm.26.2.213-215.1988.
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