Jeannot Katy, Diancourt Laure, Vaux Sophie, Thouverez Michelle, Ribeiro Amandina, Coignard Bruno, Courvalin Patrice, Brisse Sylvain
Institut Pasteur, Centre National de Référence de la Résistance aux Antibiotiques, Unité des Agents Antibactériens, Paris, France.
Institut Pasteur, Genotyping of Pathogens and Public Health, Paris, France.
PLoS One. 2014 Dec 17;9(12):e115452. doi: 10.1371/journal.pone.0115452. eCollection 2014.
Carbapenem-resistant Acinetobacter baumannii have emerged globally. The objective of this study was to investigate the epidemiology, clonal diversity and resistance mechanisms of imipenem non-susceptible A. baumannii isolates in France. Between December 2010 and August 2011, 132 notifications were collected, including 37 outbreaks corresponding to 242 cases (2 to 55 per cluster). Multilocus sequence typing, pulsed-field gel electrophoresis (PFGE) and characterisation of carbapenemase-encoding genes were performed on 110 non-repetitive isolates. Gene blaOXA-23 was the most frequently detected (82%), followed by blaOXA-24 (11%) and blaOXA-58 (7%). Eleven sequence types (ST) were distinguished, among which sequence types ST1, ST2 (64%), ST20, ST25, ST85 and ST107. Isolates from epidemiological clusters had the same ST and resistance genes, indicating probable transmission within centres. In contrast, PFGE types of isolates differed among centres, arguing against transmission among centers. This study provides the first epidemiological snapshot of the population of A. baumannii with reduced susceptibility to carbapenems from France, and further underlines the predominance of international clones.
耐碳青霉烯类鲍曼不动杆菌已在全球范围内出现。本研究的目的是调查法国对亚胺培南不敏感的鲍曼不动杆菌分离株的流行病学、克隆多样性和耐药机制。在2010年12月至2011年8月期间，收集了132份报告，包括37起暴发，涉及242例病例（每个聚集性病例为2至55例）。对110株非重复分离株进行了多位点序列分型、脉冲场凝胶电泳（PFGE）和碳青霉烯酶编码基因的鉴定。blaOXA - 23基因是最常检测到的（82%），其次是blaOXA - 24（11%）和blaOXA - 58（7%）。区分出了11种序列类型（ST），其中序列类型ST1、ST2（64%）、ST20、ST25、ST85和ST107。来自流行病学聚集性病例的分离株具有相同的ST和耐药基因，表明可能在各中心内传播。相比之下，各中心分离株的PFGE类型不同，这表明各中心之间不存在传播。本研究提供了法国对碳青霉烯类敏感性降低的鲍曼不动杆菌群体的首张流行病学概况，并进一步强调了国际克隆株的优势地位。
