Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA.
Department of Bioengineering, Northeastern University, Boston, MA 02115, USA.
Acta Biomater. 2015 Mar;14:175-84. doi: 10.1016/j.actbio.2014.12.004. Epub 2014 Dec 15.
Magnesium plays an important role in the body, mediating cell-extracellular matrix interactions and bone apatite structure and density. This study investigated, for the first time, the effects of adding magnesium oxide (MgO) nanoparticles to poly (l-lactic acid) (PLLA) and to hydroxyapatite (HA) nanoparticle-PLLA composites for orthopedic tissue engineering applications. Results showed that MgO nanoparticles significantly enhanced osteoblast adhesion and proliferation on HA-PLLA nanocomposites while maintaining mechanical properties (Young's modulus ∼1,000 MPa) suitable for cancellous bone applications. Additionally, osteoblasts (or bone-forming cells) cultured in the supernatant of degrading nanocomposites showed improved proliferation in the presence of magnesium, indicating that the increased alkalinity of solutions containing MgO nanocomposites had no toxic effects towards cells. These results together indicated the promise of further studying MgO nanoparticles as additive materials to polymers to enhance the integration of implanted biomaterials with bone.
镁在体内起着重要作用，介导细胞-细胞外基质相互作用以及骨磷灰石的结构和密度。本研究首次探讨了将氧化镁（MgO）纳米粒子添加到聚（L-乳酸）（PLLA）和纳米羟基磷灰石（HA）-PLLA 复合材料中，用于骨科组织工程应用的效果。结果表明，MgO 纳米粒子显著增强了成骨细胞在 HA-PLLA 纳米复合材料上的黏附和增殖，同时保持了适合松质骨应用的力学性能（杨氏模量约为 1000MPa）。此外，在含有镁的降解纳米复合材料的上清液中培养的成骨细胞（或成骨细胞）显示出增殖能力提高，表明含有 MgO 纳米复合材料的溶液的碱性增加对细胞没有毒性作用。这些结果共同表明，进一步研究 MgO 纳米粒子作为聚合物的添加剂材料以增强植入生物材料与骨骼的整合具有广阔的前景。
