Holder I A, Neely A N
Department of Surgery, University of Cincinnati College of Medicine, Ohio 45219.
Infect Immun. 1989 Nov;57(11):3345-8. doi: 10.1128/iai.57.11.3345-3348.1989.
Purified Pseudomonas elastase injected subcutaneously into the skin of an Evans blue dye-injected (intravenously) guinea pig caused dye leakage similar to that observed when histamine or bradykinin was injected in the same animal. The histamine-induced dye leakage was ablated in antihistamine-treated guinea pigs, but elastase- and bradykinin-induced dye leakages were not. Local injections of specific inhibitors of the host Hageman factor-dependent bradykinin-generating pathway given immediately prior to elastase injection reduced dye leakage in a dose-related manner. Elastase-related dye release was enhanced when angiotension-converting enzyme inhibitor, a substance which prevents host enzymes from breaking down bradykinin, was injected prior to elastase injection. We conclude that Pseudomonas elastase generates bradykinin in the infected host via a Hageman factor-dependent pathway.
将纯化的铜绿假单胞菌弹性蛋白酶皮下注射到预先静脉注射伊文思蓝染料的豚鼠皮肤中，会引起染料渗漏，这与在同一动物中注射组胺或缓激肽时观察到的情况相似。组胺诱导的染料渗漏在接受抗组胺治疗的豚鼠中消失，但弹性蛋白酶和缓激肽诱导的染料渗漏则没有。在弹性蛋白酶注射前立即局部注射宿主哈格曼因子依赖性缓激肽生成途径的特异性抑制剂，可使染料渗漏呈剂量相关减少。当在弹性蛋白酶注射前注射血管紧张素转换酶抑制剂（一种阻止宿主酶分解缓激肽的物质）时，弹性蛋白酶相关的染料释放会增强。我们得出结论，铜绿假单胞菌弹性蛋白酶通过哈格曼因子依赖性途径在受感染宿主中产生缓激肽。
