Baker N R
Can J Microbiol. 1982 Feb;28(2):248-55. doi: 10.1139/m82-033.
Hamster tracheal organ culture has been used to study the role of exotoxin A and proteases of Pseudomonas aeruginosa in infections of the respiratory tract. Tracheal explants infected with a toxin producing, protease producing strain or a toxin deficient, protease producing strain displayed evidence of exfoliation and disorganization of the tracheal of the tracheal epithelium 12 h after initiation of infection. A toxin producing protease deficient strain caused some desquamation of cells and cellular swelling, but exfoliation was not evident. Each of the toxin producing and (or) protease producing strains inhibited protein synthesis of the explants. Purified exotoxin inhibited protein synthesis and caused some pathological changes similar to those observed with the toxin producing strain. Purified elastase from P. aeruginosa only inhibited protein synthesis at 10 microgram/mL but caused exfoliation at 0.01 microgram/mL. Alkaline protease had no detectable effect on the explants. The effects of active infection could be prevented by treatment with gentamycin. All the strains tested caused an inhibition of ciliary activity, but no correlation could be made with any of the extracellular products. Exotoxin A and elastase may be responsible for much of the destruction of respiratory tract tissue in Pseudomonas infections although other bacterial factors and host factors are of importance.
仓鼠气管器官培养已被用于研究铜绿假单胞菌外毒素A和蛋白酶在呼吸道感染中的作用。用产毒素、产蛋白酶菌株或毒素缺陷、产蛋白酶菌株感染的气管外植体在感染开始后12小时显示出气管上皮脱落和紊乱的迹象。产毒素但蛋白酶缺陷的菌株导致一些细胞脱落和细胞肿胀，但脱落不明显。每种产毒素和（或）产蛋白酶的菌株均抑制外植体的蛋白质合成。纯化的外毒素抑制蛋白质合成，并引起一些与产毒素菌株观察到的类似的病理变化。来自铜绿假单胞菌的纯化弹性蛋白酶仅在10微克/毫升时抑制蛋白质合成，但在0.01微克/毫升时引起脱落。碱性蛋白酶对外植体没有可检测到的影响。用庆大霉素治疗可预防主动感染的影响。所有测试菌株均导致纤毛活动抑制，但与任何细胞外产物均无相关性。外毒素A和弹性蛋白酶可能是铜绿假单胞菌感染中呼吸道组织大部分破坏的原因，尽管其他细菌因素和宿主因素也很重要。
