Almutairi Meshari Saad, Ali Muhammad
a School of Civil Engineering & Surveying, University of Portsmouth , Portland Building, Portland Street, Portsmouth PO1 3AH , UK.
Nat Prod Res. 2015;29(13):1271-5. doi: 10.1080/14786419.2014.992345. Epub 2014 Dec 24.
Direct detection of saponins in soapnuts (Sapindus mukorossi) using Fourier transform infrared (FTIR) spectroscopy is investigated in this project. Potassium bromide powder was mixed with extracted powder of soapnuts and compressed to a thin pellet for examination process. The outcome of the FTIR spectra of saponin demonstrated characteristic triterpenoid saponin absorptions of OH, C = O, C-H, and C = C, while the glycoside linkages to the sapogenins were indicated by the absorptions of C-O. The significance of this study is that saponin absorption peaks are directly detectable in crude aqueous and 95% ethanol extracts of soapnuts powder using FTIR spectroscopy, thereby eliminating the need of further expensive and exhaustive purification steps. The extracts of soapnuts were screened for saponins along with controls by phytochemical tests, and advanced spectroscopic techniques such as ultra fast liquid chromatography and ultra performance liquid chromatography quadrupole-time of flight-mass spectrometry were also implemented to validate the saponins.
本项目研究了使用傅里叶变换红外（FTIR）光谱法直接检测无患子（Sapindus mukorossi）中的皂苷。将溴化钾粉末与无患子提取粉末混合，压制成薄片用于检测过程。皂苷的FTIR光谱结果显示出OH、C = O、C-H和C = C的特征三萜皂苷吸收峰，而与皂苷元的糖苷键则由C-O的吸收峰表示。本研究的意义在于，使用FTIR光谱法可直接在无患子粉末的粗水提取物和95%乙醇提取物中检测到皂苷吸收峰，从而无需进一步进行昂贵且繁琐的纯化步骤。通过植物化学测试对无患子提取物与对照品进行皂苷筛选，还采用了超快速液相色谱和超高效液相色谱四极杆-飞行时间质谱等先进光谱技术来验证皂苷。
