Boeckh M, Gleaves C A, Bindra R, Meyers J D
Fred Hutchinson Cancer Research Center, Program in Infectious Diseases, 1124 Columbia Street, Seattle, Washington 98104, USA.
Eur J Clin Microbiol Infect Dis. 1991 Jul;10(7):569-72. doi: 10.1007/BF01967276.
U-373MG astrocytoma cells are susceptible to human cytomegalovirus (CMV) infection and offer the advantage of a continuous cell line for clinical laboratory use. U-373MG to MRC-5 cells for detection of CMV by centrifugation culture were therefore compared. At 20 h, 10 (6.1%) versus 12 (7.4%) of 163 clinical specimens were positive for CMV, and at 40 h, 12 (7.4%) versus 17 (10.4%) were positive in U-373MG and MRC-5 cells, respectively. Substantial toxicity was found in U-373MG cells (84%) when inoculated with blood specimens. For detection of CMV in centrifugation culture, MRC-5 cells are superior due both to higher sensitivity and lesser toxicity.
U-373MG星形细胞瘤细胞易受人类巨细胞病毒（CMV）感染，具有可作为临床实验室使用的连续细胞系的优势。因此，比较了U-373MG细胞和MRC-5细胞通过离心培养检测CMV的情况。在20小时时，163份临床标本中分别有10份（6.1%）和12份（7.4%）在U-373MG细胞和MRC-5细胞中CMV检测呈阳性；在40小时时，分别有12份（7.4%）和17份（10.4%）呈阳性。接种血液标本后，U-373MG细胞出现大量毒性（84%）。在离心培养检测CMV时，MRC-5细胞更具优势，因为其敏感性更高且毒性更小。
