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用于离心培养中巨细胞病毒鉴定的MRC-5细胞和人包皮成纤维细胞的比较

Comparison of MRC-5 and HFF cells for the identification of cytomegalovirus in centrifugation culture.

作者信息

Gleaves C A, Meyers J D

出版信息

Diagn Microbiol Infect Dis. 1987 Feb;6(2):179-82. doi: 10.1016/0732-8893(87)90105-2.

DOI:10.1016/0732-8893(87)90105-2
PMID:3028706
Abstract

Detection of cytomegalovirus (CMV) by centrifugation culture was compared in MRC-5 and HFF cells. At 24 hr 47 of 130 specimens (36%) were positive for CMV, 45 in MRC-5, and 35 in HFF cells. At 48 hr 45 specimens were positive in both MRC-5 and HFF cells. MRC-5 cells appear to be superior for the detection of CMV at 24 hr in centrifugation culture; however, both lines are comparable at 48 hr.

摘要

在MRC - 5细胞和人包皮成纤维细胞(HFF)中,比较了通过离心培养检测巨细胞病毒(CMV)的情况。在24小时时,130个标本中有47个(36%)CMV呈阳性,其中45个在MRC - 5细胞中呈阳性,35个在HFF细胞中呈阳性。在48小时时,45个标本在MRC - 5细胞和HFF细胞中均呈阳性。在离心培养中,MRC - 5细胞在24小时时似乎更适合检测CMV;然而,在48小时时,两种细胞系的检测效果相当。

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